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LRP2-Specific Polyclonal antibody
LRP2-Specific Polyclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
Cat no : 19700-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, human brain tissue, human placenta tissue, HeLa cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:4000-1:16000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 8 publications below |
| IHC | See 4 publications below |
| IF | See 5 publications below |
Product Information
19700-1-AP targets LRP2-Specific in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | low density lipoprotein-related protein 2 |
| Calculated Molecular Weight | 522 kDa |
| Observed Molecular Weight | 280 kDa |
| GenBank Accession Number | NM_004525 |
| Gene Symbol | LRP2 |
| Gene ID (NCBI) | 4036 |
| RRID | AB_10640428 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LRP2, also named as gp330 and Megalin, belongs to the LDLR family. It acts together with cubilin to mediate HDL endocytosis. LRP2 may participate in regulation of parathyroid-hormone and para-thyroid-hormone-related protein release. Defects in LRP2 are the cause of Donnai-Barrow syndrome (DBS). This antibody is specific to LRP2 and detects endogenous LRP2 with an apparent molecular weight 280 kDa, which is smaller than the predicted molecular weight (522 kDa) and probably represent a proteolytic fragment of the large glycoprotein (PMID: 16638803; 8483924).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LRP2-Specific antibody 19700-1-AP | Download protocol |
| IHC protocol for LRP2-Specific antibody 19700-1-AP | Download protocol |
| IF protocol for LRP2-Specific antibody 19700-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EMBO Rep Amino acid starvation-induced LDLR trafficking accelerates lipoprotein endocytosis and LDL clearance. | ||
Nephrol Dial Transplant Reduced proximal tubular expression of protein endocytic receptors in proteinuria is associated with urinary receptor shedding. | ||
Development Mouse early extra-embryonic lineages activate compensatory endocytosis in response to poor maternal nutrition. | ||
Metabolism Sodium-glucose cotransporter 2 inhibition attenuates protein overload in renal proximal tubule via suppression of megalin O-GlcNacylation in progressive diabetic nephropathy. | ||
Mol Ther Nucleic Acids Gelofusine Attenuates Tubulointerstitial Injury Induced by cRGD-Conjugated siRNA by Regulating the TLR3 Signaling Pathway. | ||
Stem Cell Res Generation of a homozygous LRP2 knockout human embryonic stem cell line (FDCHDPe010-A-56) by CRISPR/Cas9 system.
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Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Brittney (Verified Customer) (11-29-2018) | Excellent antibody for IHC. Used acidic antigen retrieval on paraffin embedded mouse kidney sections. Developed with DAB substrate.
 |
FH Brittney (Verified Customer) (11-29-2018) | Excellent antibody for IHC. Used acidic antigen retrieval and developed with DAB substrate.
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