Various lysates were subjected to SDS PAGE followed by western blot with 12119-1-AP (LRDD antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 12119-1-AP (LRDD antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of L02 using 12119-1-AP
IP result of anti-LRDD (IP:12119-1-AP, 4ug; Detection:12119-1-AP 1:500) with L02 cells lysate 2800ug.
Immunohistochemical analysis of paraffin-embedded human pancreas using 12119-1-AP (LRDD antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human lung using 12119-1-AP
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12119-1-AP (LRDD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12119-1-AP (LRDD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung using 12119-1-AP
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12119-1-AP (LRDD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12119-1-AP (LRDD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 12119-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12119-1-AP (LRDD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12119-1-AP (LRDD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 12119-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12119-1-AP (LRDD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12119-1-AP (LRDD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 12119-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 12119-1-AP (LRDD antibody) at dilution of 1:50 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human pancreas using 12119-1-AP (LRDD antibody) at dilution of 1:50 (under 10x lens).
IF Staining of HeLa using 12119-1-AP
Immunofluorescent analysis of Hela cells, using LRDD antibody 12119-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Immunofluorescent analysis of Hela cells, using LRDD antibody 12119-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IF Staining of HEK-293 using 12119-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using LRDD antibody (12119-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using LRDD antibody (12119-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human lung tissue, human pancreas tissue, human liver tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HEK-293 cells, HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:1000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
P53-induced protein with a death domain (PIDD/LRDD) is a component of the PIDDosome. PIDD contains 910 residues with 7 leucine rich repeats (LRRs), 2 ZU-5 domains and a C-terminal death domain (DD). PIDD can be cleaved into shorter fragments generating a PIDD-N fragment of 48 kD (residues 1-446), a PIDD-C fragment of 51 kD (residues 447-910) and a PIDD-CC fragment of 37 kD (residues 589-910). Auto-cleavage of PIDD determines the downstream signaling events. The PIDD-C fragment mediates activation of NFκB via the recruitment of RIP1 and NEMO, while PIDD-CC causes caspase-2 activation, which leads to apoptosis.
Various lysates were subjected to SDS PAGE followed by western blot with 12119-1-AP (LRDD antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human pancreas using 12119-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 12119-1-AP (LRDD antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human lung using 12119-1-AP
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12119-1-AP (LRDD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung using 12119-1-AP
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12119-1-AP (LRDD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 12119-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12119-1-AP (LRDD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 12119-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12119-1-AP (LRDD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 12119-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 12119-1-AP (LRDD antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 12119-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 12119-1-AP (LRDD antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human pancreas using 12119-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 12119-1-AP (LRDD antibody) at dilution of 1:50 (under 10x lens).
IP Figures
IP experiment of L02 using 12119-1-AP
IP result of anti-LRDD (IP:12119-1-AP, 4ug; Detection:12119-1-AP 1:500) with L02 cells lysate 2800ug.
IF/ICC Figures
IF Staining of HeLa using 12119-1-AP
Immunofluorescent analysis of Hela cells, using LRDD antibody 12119-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IF Staining of HEK-293 using 12119-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using LRDD antibody (12119-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
LRDD Polyclonal antibody
Catalog Number
12119-1-AP
Citations
2
Dilutions
WB : 1:500-1:1000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:250-1:1000 IF/ICC : 1:200-1:800
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
