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LRDD Polyclonal antibody

LRDD Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12119-1-AP

Synonyms

PIDD-C, PIDD1, PIDD, p53-induced death domain-containing protein 1, Leucine-rich repeat and death domain-containing protein

Validation Data Gallery

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  • Western Blot (WB) analysis of L02 cells using LRDD Polyclonal antibody (12119-1-AP)

    WB analysis of L02 using 12119-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 12119-1-AP (LRDD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using LRDD Polyclonal antibody (12119-1-AP)

    WB analysis of HEK-293 using 12119-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12119-1-AP (LRDD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using LRDD Polyclonal antibody (12119-1-AP)

    WB analysis of L02 using 12119-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 12119-1-AP (LRDD Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of L02 cells using LRDD Polyclonal antibody (12119-1-AP)

    IP experiment of L02 using 12119-1-AP

    IP result of anti-LRDD (IP:12119-1-AP, 4ug; Detection:12119-1-AP 1:500) with L02 cells lysate 2800ug.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using LRDD Polyclonal antibody (12119-1-AP)

    IHC staining of human pancreas using 12119-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 12119-1-AP (LRDD antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using LRDD Polyclonal antibody (12119-1-AP)

    IHC staining of human lung using 12119-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12119-1-AP (LRDD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using LRDD Polyclonal antibody (12119-1-AP)

    IHC staining of human lung using 12119-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12119-1-AP (LRDD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using LRDD Polyclonal antibody (12119-1-AP)

    IHC staining of human lung cancer using 12119-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12119-1-AP (LRDD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using LRDD Polyclonal antibody (12119-1-AP)

    IHC staining of human lung cancer using 12119-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12119-1-AP (LRDD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using LRDD Polyclonal antibody (12119-1-AP)

    IHC staining of human liver using 12119-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 12119-1-AP (LRDD antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using LRDD Polyclonal antibody (12119-1-AP)

    IHC staining of human liver using 12119-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 12119-1-AP (LRDD antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using LRDD Polyclonal antibody (12119-1-AP)

    IHC staining of human pancreas using 12119-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 12119-1-AP (LRDD antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using LRDD Polyclonal antibody (12119-1-AP)

    IF Staining of HeLa using 12119-1-AP

    Immunofluorescent analysis of Hela cells, using LRDD antibody 12119-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using LRDD Polyclonal antibody (12119-1-AP)

    IF Staining of HEK-293 using 12119-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using LRDD antibody (12119-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Tested Applications

Positive WB detected inL02 cells, HEK-293 cells
Positive IP detected inL02 cells
Positive IHC detected inhuman lung tissue, human pancreas tissue, human liver tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

12119-1-AP targets LRDD in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LRDD fusion protein Ag2761
Full Name leucine-rich repeats and death domain containing
Calculated Molecular Weight 893 aa, 98 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC014904
Gene Symbol LRDD
Gene ID (NCBI) 55367
RRIDAB_10694424
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

P53-induced protein with a death domain (PIDD/LRDD) is a component of the PIDDosome. PIDD contains 910 residues with 7 leucine rich repeats (LRRs), 2 ZU-5 domains and a C-terminal death domain (DD). PIDD can be cleaved into shorter fragments generating a PIDD-N fragment of 48 kD (residues 1-446), a PIDD-C fragment of 51 kD (residues 447-910) and a PIDD-CC fragment of 37 kD (residues 589-910). Auto-cleavage of PIDD determines the downstream signaling events. The PIDD-C fragment mediates activation of NFκB via the recruitment of RIP1 and NEMO, while PIDD-CC causes caspase-2 activation, which leads to apoptosis.

Protocols

Product Specific Protocols
WB protocol for LRDD antibody 12119-1-APDownload protocol
IHC protocol for LRDD antibody 12119-1-APDownload protocol
IF protocol for LRDD antibody 12119-1-APDownload protocol
IP protocol for LRDD antibody 12119-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Neural Regen Res

Neat1 decreases neuronal apoptosis after oxygen and glucose deprivation.

Authors - Wei-Na Chai
View Article
mouseWB

Exp Neurol

Microglia aggravate white matter injury via C3/C3aR pathway after experimental subarachnoid hemorrhage

Authors - Lei Yang
View Article