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LRCH3 Polyclonal antibody

LRCH3 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15800-1-AP

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of human liver tissue using LRCH3 Polyclonal antibody (15800-1-AP)

    WB analysis of human liver using 15800-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 15800-1-AP (LRCH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using LRCH3 Polyclonal antibody (15800-1-AP)

    WB analysis of Jurkat using 15800-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15800-1-AP (LRCH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using LRCH3 Polyclonal antibody (15800-1-AP)

    WB analysis of human heart using 15800-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 15800-1-AP (LRCH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human spleen tissue using LRCH3 Polyclonal antibody (15800-1-AP)

    WB analysis of human spleen using 15800-1-AP

    human spleen tissue were subjected to SDS PAGE followed by western blot with 15800-1-AP (LRCH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using LRCH3 Polyclonal antibody (15800-1-AP)

    WB analysis of mouse lung using 15800-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 15800-1-AP (LRCH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using LRCH3 Polyclonal antibody (15800-1-AP)

    IP experiment of mouse liver using 15800-1-AP

    IP result of anti-LRCH3 (IP:15800-1-AP, 3ug; Detection:15800-1-AP 1:1000) with mouse liver tissue lysate 12500ug.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using LRCH3 Polyclonal antibody (15800-1-AP)

    IF Staining of A431 using 15800-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using LRCH3 antibody (15800-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman liver tissue, human heart tissue, human spleen tissue, Jurkat cells, mouse lung tissue
Positive IP detected inmouse liver tissue
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15800-1-AP targets LRCH3 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LRCH3 fusion protein Ag8511
Full Name leucine-rich repeats and calponin homology (CH) domain containing 3
Calculated Molecular Weight 86 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC007504
Gene Symbol LRCH3
Gene ID (NCBI) 84859
RRIDAB_2138461
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LRCH3 antibody 15800-1-APDownload protocol
IF protocol for LRCH3 antibody 15800-1-APDownload protocol
IP protocol for LRCH3 antibody 15800-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols