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LRCH2 Polyclonal antibody

LRCH2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24866-1-AP

Synonyms

dA204F4.4, KIAA1495, LRCH2

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using LRCH2 Polyclonal antibody (24866-1-AP)

    WB analysis of mouse brain using 24866-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24866-1-AP (LRCH2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse ovary tissue using LRCH2 Polyclonal antibody (24866-1-AP)

    WB analysis of mouse ovary using 24866-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 24866-1-AP (LRCH2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using LRCH2 Polyclonal antibody (24866-1-AP)

    WB analysis of mouse heart using 24866-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 24866-1-AP (LRCH2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using LRCH2 Polyclonal antibody (24866-1-AP)

    IHC staining of human colon using 24866-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24866-1-AP (LRCH2 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using LRCH2 Polyclonal antibody (24866-1-AP)

    IHC staining of human colon using 24866-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24866-1-AP (LRCH2 Antibody) at dilution of 1:50 (under 10x lens).

Tested Applications

Positive WB detected inmouse brain tissue, mouse heart tissue, mouse ovary tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24866-1-AP is LRCH2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LRCH2 fusion protein Ag21701
Full Name leucine-rich repeats and calponin homology (CH) domain containing 2
Calculated Molecular Weight 765 aa, 85 kDa
Observed Molecular Weight 84 kDa
GenBank Accession NumberBC125224
Gene Symbol LRCH2
Gene ID (NCBI) 57631
RRIDAB_2879764
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Leucine rich repeat and calponin homology domain containing protein 2 (LRCH2) also named as KIAA1495 is a 765 amino acid protein, which contains one CH domain and nine LRR repeats. The function of LRCH2 is still non-known.

Protocols

Product Specific Protocols
WB protocol for LRCH2 antibody 24866-1-APDownload protocol
IHC protocol for LRCH2 antibody 24866-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols