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LRCH1 Polyclonal antibody

LRCH1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 25017-1-AP

Synonyms

Neuronal protein 81, Leucine-rich repeat and calponin homology domain-containing protein 1, KIAA1016, CHDC1, Calponin homology domain-containing protein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using LRCH1 Polyclonal antibody (25017-1-AP)

    WB analysis of HeLa using 25017-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25017-1-AP (LRCH1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using LRCH1 Polyclonal antibody (25017-1-AP)

    IP experiment of HeLa using 25017-1-AP

    IP result of anti-LRCH1 (IP:25017-1-AP, 4ug; Detection:25017-1-AP 1:300) with HeLa cells lysate 1600ug.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using LRCH1 Polyclonal antibody (25017-1-AP)

    IHC staining of mouse cerebellum using 25017-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25017-1-AP (LRCH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using LRCH1 Polyclonal antibody (25017-1-AP)

    IHC staining of mouse cerebellum using 25017-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25017-1-AP (LRCH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using LRCH1 Polyclonal antibody (25017-1-AP)

    IF Staining of MCF-7 using 25017-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using LRCH1 antibody (25017-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25017-1-AP targets LRCH1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LRCH1 fusion protein Ag18728
Full Name leucine-rich repeats and calponin homology (CH) domain containing 1
Calculated Molecular Weight 728 aa, 80 kDa
Observed Molecular Weight 85-95 kDa
GenBank Accession NumberBC112937
Gene Symbol LRCH1
Gene ID (NCBI) 23143
RRIDAB_2879848
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LRCH1, also named as CHDC1 or Neuronal protein 81, is a 728 amino acid protein, which contains one CH domain and nine LRR repeats. The molecular weight of LRCH1 is 81 kDa and it contains phosphorylation modified residues. we detected a 85-95 kDa protein by western blot. The function of LRCH1 is still non-known.

Protocols

Product Specific Protocols
WB protocol for LRCH1 antibody 25017-1-APDownload protocol
IHC protocol for LRCH1 antibody 25017-1-APDownload protocol
IF protocol for LRCH1 antibody 25017-1-APDownload protocol
IP protocol for LRCH1 antibody 25017-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols