LRAT Polyclonal antibody
LRAT Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, ELISA
Conjugate
Unconjugated
Cat no : 12815-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse testis tissue, Y79 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
Product Information
12815-1-AP targets LRAT in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | LRAT fusion protein Ag3556 |
| Full Name | lecithin retinol acyltransferase (phosphatidylcholine--retinol O-acyltransferase) |
| Calculated Molecular Weight | 230 aa, 26 kDa |
| Observed Molecular Weight | 26 kDa, 50-54 kDa |
| GenBank Accession Number | BC031053 |
| Gene Symbol | LRAT |
| Gene ID (NCBI) | 9227 |
| RRID | AB_2297180 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LRAT belongs to the H-rev107 family. LRAT transfers the acyl group from the sn-1 position of phosphatidylcholine to all-trans retinol, producing all-trans retinyl esters. LRAT plays a critical role in vision. It provides the all-trans retinyl ester substrates for the isomerohydrolase which processes the esters into 11-cis-retinol in the retinal pigment epithelium; due to a membrane-associated alcohol dehydrogenase, LRAT monomers (26kDa) interact in membranes and form functional homodimers (50-54 kDa) through protein-protein interactions and disulfide bond formation.Defects in LRAT are a cause of severe early-onset retinal dystrophy (RD). This antibody was generated against the internal residues of human LRAT. It is predicted to detect endogenous LRAT.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LRAT antibody 12815-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Food Biochem Inhibitory effect of chitooligosaccharides on retinol metabolism and bioavailability in mice. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Peter (Verified Customer) (12-03-2018) | The Proteintech LRAT primary antibody works so well and the antibody is prepared using BSA it can be use repeatedly when stored at -20. The strength of the antibody remains the same over a period of 6 months or more.
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