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LPL Polyclonal antibody

LPL Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 16899-1-AP

Synonyms

HDLCQ11, LIPD, lipoprotein lipase, LPL

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using LPL Polyclonal antibody (16899-1-AP)

    WB analysis of MCF-7 using 16899-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 16899-1-AP (LPL Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using LPL Polyclonal antibody (16899-1-AP)

    WB analysis of mouse skeletal muscle using 16899-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16899-1-AP (LPL Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using LPL Polyclonal antibody (16899-1-AP)

    WB analysis of HeLa using 16899-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16899-1-AP (LPL Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells, mouse skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 16899-1-AP is LPL Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LPL fusion protein Ag10431
Full Name lipoprotein lipase
Calculated Molecular Weight 475 aa, 53 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC011353
Gene Symbol LPL
Gene ID (NCBI) 4023
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LPL(lipoprotein lipase) is also named as LIPD and belongs to the AB hydrolase superfamily. LPL catalyses the hydrolysis of the triacylglycerol component of circulating chylomicrons and very low density lipoproteins. It plays a role in the binding of lipoprotein particles to cell-surface molecules, including sulfated glycosaminoglycans (GAGs)(PMID:21177248). The gene encodes a protein of 475 amino acids that becomes a mature protein of 448 residues after cleavage of a signal peptide. This protein is highly glycosylated and functions as a head-to-tail dimer under well-defined conditions(PMID:11154699).

Publications

SpeciesApplicationTitle
mouseWB

Diabetes

Rab8a Deficiency in Skeletal Muscle Causes Hyperlipidemia and Hepatosteatosis Via Impairment of Muscle Lipid Uptake and Storage.

Authors - Qiaoli Chen
View Article

Diabetes

Tissue-specific splicing and dietary interaction of a mutant As160 allele determine muscle metabolic fitness in rodents.

Authors - Xinyu Yang
View Article
mouseWB

Biology (Basel)

Curcumin-Rich Diet Mitigates Non-Alcoholic Fatty Liver Disease (NAFLD) by Attenuating Fat Accumulation and Improving Insulin Sensitivity in Aged Female Mice under Nutritional Stress

Authors - Gopal Lamichhane
View Article