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LPCAT2 Polyclonal antibody

LPCAT2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15082-1-AP

Synonyms

1-alkylglycerophosphocholine O-acetyltransferase, 1-alkenylglycerophosphocholine O-acyltransferase, 1-AGPAT 11, 1-AGP acyltransferase 11, 1-acylglycerophosphocholine O-acyltransferase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LPCAT2 Polyclonal antibody (15082-1-AP)

    WB analysis using 15082-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15082-1-AP (LPCAT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using LPCAT2 Polyclonal antibody (15082-1-AP)

    WB analysis of HeLa using 15082-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15082-1-AP (LPCAT2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of LPS treated HEK-293 using LPCAT2 Polyclonal antibody (15082-1-AP)

    WB analysis of LPS treated HEK-293 using 15082-1-AP

    LPS treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 15082-1-AP (LPCAT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using LPCAT2 Polyclonal antibody (15082-1-AP)

    IF Staining of A431 using 15082-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using LPCAT2 antibody (15082-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inHEK-293 cells, LPS treated HEK-293, HeLa cells, Jurkat cells, RAW 264.7 cells
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15082-1-AP targets LPCAT2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LPCAT2 fusion protein Ag7156
Full Name lysophosphatidylcholine acyltransferase 2
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC002472
Gene Symbol LPCAT2
Gene ID (NCBI) 54947
RRIDAB_2135557
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LPCAT2 antibody 15082-1-APDownload protocol
IF protocol for LPCAT2 antibody 15082-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Antioxidants (Basel)

Mitochondrial Reactive Oxygen Species Formation Determines ACSL4/LPCAT2-Mediated Ferroptosis

Authors - Melanie Merkel
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Melanie (Verified Customer) (04-22-2022)

LPCAT2 antibody was diluted 1:1500 in 5% milk in TBST and applied to protein samples from Hek293T cells. The bands were very clear. The antibody could have been diluted even more.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1500
  • Cell Tissue Type: Hek293T cells
FH

Christian (Verified Customer) (12-17-2018)

10 μg protein per lane run on 10% Tris-Glycine gel. Primary incubation 1:400 overnight @ 4°C. Secondary antibody and biotinylated marker were supplied from a different vendor and used and used at optimized dilutions.LPCAT2 siRNA was used to check antibody specificity.The antibody performed excellently, will try at a dilution of 1:800 next time.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:400
  • Cell Tissue Type: RAW264.7
LPCAT2 Antibody Western Blot, validation (1:400 dilution) in RAW264.7 (Cat no:15082-1-AP)