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LOC286135 Polyclonal antibody

LOC286135 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21958-1-AP

Synonyms

hypothetical LOC286135, LOC286135

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using LOC286135 Polyclonal antibody (21958-1-AP)

    WB analysis of mouse brain using 21958-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21958-1-AP (LOC286135 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using LOC286135 Polyclonal antibody (21958-1-AP)

    WB analysis of mouse brain using 21958-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21958-1-AP (LOC286135 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using LOC286135 Polyclonal antibody (21958-1-AP)

    IHC staining of human testis using 21958-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 21958-1-AP (LOC286135 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using LOC286135 Polyclonal antibody (21958-1-AP)

    IHC staining of human testis using 21958-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 21958-1-AP (LOC286135 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using LOC286135 Polyclonal antibody (21958-1-AP)

    IHC staining of human liver using 21958-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 21958-1-AP (LOC286135 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using LOC286135 Polyclonal antibody (21958-1-AP)

    IHC staining of human liver using 21958-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 21958-1-AP (LOC286135 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman testis tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21958-1-AP is LOC286135 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LOC286135 fusion protein Ag16644
Full Name hypothetical LOC286135
Calculated Molecular Weight 138 aa, 15 kDa
Observed Molecular Weight 18-20 kDa
GenBank Accession NumberBC126344
Gene Symbol LOC286135
Gene ID (NCBI) 286135
RRIDAB_11042462
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LOC286135 antibody 21958-1-APDownload protocol
IHC protocol for LOC286135 antibody 21958-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols