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LNPEP Polyclonal antibody

LNPEP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55154-1-AP

Synonyms

CAP, Cystinyl aminopeptidase, IRAP, Leucyl cystinyl aminopeptidase, leucyl/cystinyl aminopeptidase, LNPEP, OTase, Oxytocinase, P LAP, PLAP, VP165

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using LNPEP Polyclonal antibody (55154-1-AP)

    WB analysis of human brain using 55154-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 55154-1-AP (LNPEP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using LNPEP Polyclonal antibody (55154-1-AP)

    WB analysis of human heart using 55154-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 55154-1-AP (LNPEP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using LNPEP Polyclonal antibody (55154-1-AP)

    WB analysis of COLO 320 using 55154-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 55154-1-AP (LNPEP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using LNPEP Polyclonal antibody (55154-1-AP)

    WB analysis of human brain using 55154-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 55154-1-AP (LNPEP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using LNPEP Polyclonal antibody (55154-1-AP)

    IHC staining of human heart using 55154-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 55154-1-AP (LNPEP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using LNPEP Polyclonal antibody (55154-1-AP)

    IHC staining of human heart using 55154-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 55154-1-AP (LNPEP antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using LNPEP Polyclonal antibody (55154-1-AP)

    IHC staining of human kidney using 55154-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 55154-1-AP (LNPEP antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using LNPEP Polyclonal antibody (55154-1-AP)

    IHC staining of human kidney using 55154-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 55154-1-AP (LNPEP antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using LNPEP Polyclonal antibody (55154-1-AP)

    IHC staining of human kidney using 55154-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 55154-1-AP (LNPEP antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using LNPEP Polyclonal antibody (55154-1-AP)

    IHC staining of human kidney using 55154-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 55154-1-AP (LNPEP antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inhuman brain tissue, COLO 320 cells, human heart tissue
Positive IHC detected inhuman heart tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55154-1-AP targets LNPEP in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name leucyl/cystinyl aminopeptidase
Calculated Molecular Weight 117 kDa
Observed Molecular Weight 117-125 kDa
GenBank Accession NumberNM_005575
Gene Symbol LNPEP
Gene ID (NCBI) 4012
RRIDAB_10860904
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LNPEP, also named as OTASE, IRAP, P-LAP and Otase, may be involved in the inactivation of neuronal peptides in the brain. LNPEP cleaves Met-enkephalin and dynorphin. It binds angiotensin IV and may be the angiotensin IV receptor in the brain. It can be detected a protein with molecular mass of 165 kDa in several human tissues including heart, small intestine, kidney and 140 kDa in brain. LNPEP contains 18 potential N-glycosylation sites so that the differential usage of the glycosylation site in the brain causes the difference in the molecular mass of the enzyme.(PMID:11389728). Two forms of LNPEP are purified from human placenta: one is a monomeric, 61-kDa protein and the other is a heterodimeric protein with 64- and 97-kDa subunits(PMID:10601849). It can form a homodimer of 280 kDa(PMID:10601849). This antibody is specific to LNPEP.

Protocols

Product Specific Protocols
WB protocol for LNPEP antibody 55154-1-APDownload protocol
IHC protocol for LNPEP antibody 55154-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols