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- KD/KO Validated
LMOD1 Polyclonal antibody
LMOD1 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
Cat no : 15117-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse bladder tissue, mouse uterus tissue, HT-29 cells |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 13 publications below |
| IHC | See 4 publications below |
| IF | See 9 publications below |
Product Information
15117-1-AP targets LMOD1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | LMOD1 fusion protein Ag7176 |
| Full Name | leiomodin 1 (smooth muscle) |
| Calculated Molecular Weight | 67 kDa |
| Observed Molecular Weight | 85 kDa, 64 kDa |
| GenBank Accession Number | BC001755 |
| Gene Symbol | LMOD1 |
| Gene ID (NCBI) | 25802 |
| RRID | AB_2136682 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LMOD1 was initially identified as a 64-kDa autoantigen in an expression screen with sera from patients with Hashimoto's thyroiditis. Recently immunohistochemistry and immunoblotting of LMOD1 demonstrated its highly restrictive pattern of expression in SMC lineages,like adult aorta, and bladder. LMOD1 is also shown to exhibit an embryonic and adult expression pattern. These data identify LMOD1 as a new SMC-restricted gene associated with the SMC differentiated phenotype.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LMOD1 antibody 15117-1-AP | Download protocol |
| IHC protocol for LMOD1 antibody 15117-1-AP | Download protocol |
| IF protocol for LMOD1 antibody 15117-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Gastroenterology The Long Non-coding RNA Carmn is a Critical Regulator of Gastrointestinal Smooth Muscle Contractile Function and Motility | ||
Nat Genet Single-nucleus chromatin accessibility profiling highlights regulatory mechanisms of coronary artery disease risk. | ||
Sci Transl Med Nodding syndrome may be an autoimmune reaction to the parasitic worm Onchocerca volvulus. | ||
Genome Biol Prime editing in mice reveals the essentiality of a single base in driving tissue-specific gene expression. | ||
Proc Natl Acad Sci U S A Loss of LMOD1 impairs smooth muscle cytocontractility and causes megacystis microcolon intestinal hypoperistalsis syndrome in humans and mice.
| ||
PLoS Genet Functional regulatory mechanism of smooth muscle cell-restricted LMOD1 coronary artery disease locus.
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