Lamin B1 AntibodyRabbit Polyclonal
|Tested applications||ELISA, IHC, IF, IP, WB, FC|
|Species specificity||Human,Mouse,Rat; other species not tested.|
|Cited species||human, mouse, rat|
|Positive WB detected in||HeLa cells, HepG2 cells, human brain tissue, human kidney tissue, Jurkat cells, K-562 cells, mouse brain tissue, mouse eye tissue, mouse kidney tissue, mouse lung tissue, mouse pancreas tissue, mouse spleen tissue, NIH/3T3 cells, rat brain tissue, SH-SY5Y cells, U-937 cells, Y79 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human breast cancer tissue, human kidney tissue|
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HEK-293 cells|
|Recommended dilution||WB: 1:500-1:5000|
IP = Immunoprecipitation
- HeLa cells were subjected to SDS PAGE followed by western blot with 12987-1-AP(LMNB1 antibody) at dilution of 1:1000
- Immunofluorescent analysis of HepG2 cells, using LMNB1 antibody 12987-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Immunohistochemical of paraffin-embedded human breast cancer using 12987-1-AP(LMNB1 antibody) at dilution of 1:100 (under 10x lens)
- 1X10^6 HEK-293 cells were stained with 0.2ug Lamin B1 antibody (12987-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat anti-Rabbit IgG with dilution 1:100.
- IP Result of anti-LMNB1 (IP:12987-1-AP, 3ug; Detection:12987-1-AP 1:1000) with HeLa cells lysate 2500ug.
|Source||Rabbit||Purification method||Antigen affinity purification|
|Isotype||IgG||Storage||PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.|
|Immunogen||Lamin B1 fusion protein ag3631||Full name||lamin B1|
|BC012295||Gene ID (NCBI)||4001|
|Gene symbol||LMNB1||Synonyms||ADLD, lamin B1, LMN, LMN2, LMNB, LMNB1|
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Expression of uncleavable mutant lamin A or B caused significant delays in the onset of chromatin condensation and nuclear shrinkage during apoptosis(PMID:11953316). This protein is not suitable for samples where the nuclear envelope has been removed.