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LIPI Polyclonal antibody

LIPI Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24300-1-AP

Synonyms

Cancer/testis antigen 17, CT17, Lipase member I, lipase, member I, LIPI, LPD lipase, LPDL, mPA PLA1 beta, PRED5

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using LIPI Polyclonal antibody (24300-1-AP)

    WB analysis of A549 using 24300-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 24300-1-AP (LIPI Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using LIPI Polyclonal antibody (24300-1-AP)

    WB analysis of mouse lung using 24300-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 24300-1-AP (LIPI Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using LIPI Polyclonal antibody (24300-1-AP)

    IHC staining of human testis using 24300-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24300-1-AP (LIPI antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using LIPI Polyclonal antibody (24300-1-AP)

    IHC staining of human testis using 24300-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24300-1-AP (LIPI antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inA549 cells, mouse lung tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24300-1-AP is LIPI Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LIPI fusion protein Ag19024
Full Name lipase, member I
Calculated Molecular Weight 460 aa, 55 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC148691
Gene Symbol LIPI
Gene ID (NCBI) 149998
RRIDAB_2879483
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LIPI antibody 24300-1-APDownload protocol
IHC protocol for LIPI antibody 24300-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols