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LIN7C Polyclonal antibody

LIN7C Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14656-1-AP

Synonyms

VELI3, Protein lin-7 homolog C, Mammalian lin-seven protein 3, MALS-3, MALS3

Validation Data Gallery

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  • Western Blot (WB) analysis of human cerebellum tissue using LIN7C Polyclonal antibody (14656-1-AP)

    WB analysis of human cerebellum using 14656-1-AP

    human cerebellum tissue were subjected to SDS PAGE followed by western blot with 14656-1-AP (LIN7C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using LIN7C Polyclonal antibody (14656-1-AP)

    WB analysis of human brain using 14656-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 14656-1-AP (LIN7C antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using LIN7C Polyclonal antibody (14656-1-AP)

    WB analysis of human kidney using 14656-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 14656-1-AP (LIN7C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using LIN7C Polyclonal antibody (14656-1-AP)

    IHC staining of human stomach using 14656-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14656-1-AP (LIN7C antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using LIN7C Polyclonal antibody (14656-1-AP)

    IF Staining of HeLa using 14656-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14656-1-AP (LIN7C antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman cerebellum tissue, human brain tissue, human kidney tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

14656-1-AP targets LIN7C in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LIN7C fusion protein Ag6265
Full Name lin-7 homolog C (C. elegans)
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC053907
Gene Symbol LIN7C
Gene ID (NCBI) 55327
RRIDAB_2135197
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LIN7C antibody 14656-1-APDownload protocol
IHC protocol for LIN7C antibody 14656-1-APDownload protocol
IF protocol for LIN7C antibody 14656-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Neuroscience

Proteome analysis of actin filament-associated proteins in the postnatal rat cerebellum.

Authors - Shi N N
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Thomas (Verified Customer) (11-17-2020)

Good specifity, also binds canine Lin7c

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HCT116 (human, colon cancer cell line), Caco2 (human, colorectal adenocarcinoma cell line) , MDCK (canine, kidney tubule)
LIN7C Antibody Western Blot validation (1:1000 dilution) in HCT116 (human, colon cancer cell line), Caco2 (human, colorectal adenocarcinoma cell line) , MDCK (canine, kidney tubule) (Cat no:14656-1-AP)