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LIN7A Polyclonal antibody

LIN7A Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25150-1-AP

Synonyms

hLin 7, lin 7 homolog A (C. elegans), LIN 7A, LIN7, LIN7A, MALS 1, MALS1, Mammalian lin seven protein 1, Protein lin 7 homolog A, Tax interaction protein 33, TIP 33, Veli 1, VELI1, Vertebrate lin 7 homolog 1

Validation Data Gallery

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  • Western Blot (WB) analysis of rat brain tissue using LIN7A Polyclonal antibody (25150-1-AP)

    WB analysis of rat brain using 25150-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 25150-1-AP (LIN7A Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using LIN7A Polyclonal antibody (25150-1-AP)

    IP experiment of mouse brain using 25150-1-AP

    IP result of anti-LIN7A (IP:25150-1-AP, 3ug; Detection:25150-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using LIN7A Polyclonal antibody (25150-1-AP)

    IHC staining of mouse brain using 25150-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25150-1-AP (LIN7A Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using LIN7A Polyclonal antibody (25150-1-AP)

    IHC staining of mouse brain using 25150-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25150-1-AP (LIN7A Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inrat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

The immunogen of 25150-1-AP is LIN7A Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LIN7A fusion protein Ag18440
Full Name lin-7 homolog A (C. elegans)
Calculated Molecular Weight 233 aa, 26 kDa
Observed Molecular Weight 28-30 kDa
GenBank Accession NumberBC118609
Gene Symbol LIN7A
Gene ID (NCBI) 8825
RRIDAB_2879925
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LIN7A antibody 25150-1-APDownload protocol
IHC protocol for LIN7A antibody 25150-1-APDownload protocol
IP protocol for LIN7A antibody 25150-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIF

iScience

Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration

Authors - Haibin Tian
View Article