• Featured Product
  • KD/KO Validated

DNA Ligase I Polyclonal antibody

DNA Ligase I Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 18051-1-AP

Synonyms

DNA ligase 1, DNA Ligase I, LIG1, ligase I, DNA, ATP dependent



Tested Applications

Positive WB detected inHeLa cells, A431 cells, Jurkat cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman spleen tissue, human brain tissue, human placenta tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18051-1-AP targets DNA Ligase I in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNA Ligase I fusion protein Ag12508
Full Name ligase I, DNA, ATP-dependent
Calculated Molecular Weight 919 aa, 102 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC108318
Gene Symbol LIG1
Gene ID (NCBI) 3978
RRIDAB_2265726
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNA ligase I (LIG1) joins DNA strand breaks during DNA replication and repair transactions and contributes to genome integrity. 18051-1-AP is raised agains the C-terminal 670-919 aa residues of the human DNA ligase 1.

Protocols

Product Specific Protocols
WB protocol for DNA Ligase I antibody 18051-1-APDownload protocol
IHC protocol for DNA Ligase I antibody 18051-1-APDownload protocol
IP protocol for DNA Ligase I antibody 18051-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

eccDNAs are apoptotic products with high innate immunostimulatory activity

Authors - Yuangao Wang
humanWB,IHC

Mol Cancer

hsa_circ_0007919 induces LIG1 transcription by binding to FOXA1/TET1 to enhance the DNA damage response and promote gemcitabine resistance in pancreatic ductal adenocarcinoma

Authors - Lei Xu
mouseWB

Nat Commun

Cas9-induced large deletions and small indels are controlled in a convergent fashion.

Authors - Michael Kosicki
humanWB,IP

J Clin Invest

Biallelic mutations in DNA ligase 1 underlie a spectrum of immune deficiencies.

Authors - Patrick Maffucci
  • KO Validated
ratWB

Proc Natl Acad Sci U S A

Redundant function of DNA ligase 1 and 3 in alternative end-joining during immunoglobulin class switch recombination.

Authors - Shahnaz Masani
  • KO Validated
mouseWB

Proc Natl Acad Sci U S A

Ligase I and ligase III mediate the DNA double-strand break ligation in alternative end-joining.

Authors - Guangqing Lu
  • KO Validated