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LHPP Polyclonal antibody

Name: LHPP Polyclonal antibody
Brand: Proteintech
SKU: 15759-1-AP

LHPP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 15759-1-AP

Synonyms

Phospholysine phosphohistidine inorganic pyrophosphate phosphatase, hLHPP, EC:3.1.3.-

Validation Data Gallery

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AllWBIHCIF/ICC

WB analysis using 15759-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15759-1-AP (LHPP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 15759-1-AP
WB result of LHPP antibody (15759-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LHPP transfected A549 cells.
WB analysis of mouse liver using 15759-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 15759-1-AP (LHPP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 15759-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15759-1-AP (LHPP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 15759-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 15759-1-AP (LHPP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 15759-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 15759-1-AP (LHPP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 15759-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 15759-1-AP (LHPP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 15759-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15759-1-AP (LHPP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 15759-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15759-1-AP (LHPP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 15759-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 15759-1-AP (LHPP antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human kidney using 15759-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 15759-1-AP (LHPP antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HeLa using 15759-1-AP
Immunofluorescent analysis of Hela cells, using LHPP antibody 15759-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Cat No. 15759-1-AP

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Tested Applications

Positive WB detected in	mouse liver tissue, human liver tissue, human brain tissue, A549 cells, HeLa cells, rat liver tissue, A431 cells, HepG2 cells
Positive IHC detected in	human liver cancer tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Hela cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 13 publications below
IHC	See 7 publications below
IF	See 2 publications below

Product Information

15759-1-AP targets LHPP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	LHPP fusion protein Ag8375
Full Name	phospholysine phosphohistidine inorganic pyrophosphate phosphatase
Calculated Molecular Weight	23 kDa, 29 kDa
Observed Molecular Weight	23-32 kDa
GenBank Accession Number	BC007324
Gene Symbol	LHPP
Gene ID (NCBI)	64077
RRID	AB_2296904
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LHPP antibody 15759-1-AP	Download protocol
IHC protocol for LHPP antibody 15759-1-AP	Download protocol
IF protocol for LHPP antibody 15759-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Mol Cancer YTHDF2 mediates the mRNA degradation of the tumor suppressors to induce AKT phosphorylation in N6-methyladenosine-dependent way in prostate cancer. Authors - Jiangfeng Li View Article
human	WB,IHC	Cell Death Dis Phosphatase LHPP confers prostate cancer ferroptosis activation by modulating the AKT-SKP2-ACSL4 pathway Authors - Guoqing Xie View Article
human	WB	Biochim Biophys Acta Mol Basis Dis Correlated with better prognosis, CSTA inhibits metastasis of nasopharyngeal carcinoma cells via suppressing AKT signaling through promoting METTL3 degradation Authors - Zhi-Jie Liu View Article
human	WB	Aging (Albany NY) The long intergenic noncoding RNA GAS5 reduces cisplatin-resistance in non-small cell lung cancer through the miR-217/LHPP axis. Authors - Xuhui Yang KD Validated View Article
human	WB	Cell Cycle LHPP inhibits hepatocellular carcinoma cell growth and metastasis. Authors - Lijuan Liao View Article
human	WB,IHC,IF	Oncol Rep Tumor suppressor LHPP regulates the proliferation of colorectal cancer cells via the PI3K/AKT pathway. Authors - Bin Hou View Article

LHPP Polyclonal antibody

LHPP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 15759-1-AP

WB analysis of A549 using 15759-1-AP

WB analysis of mouse liver using 15759-1-AP

WB analysis using 15759-1-AP

WB analysis of human liver using 15759-1-AP

WB analysis of human brain using 15759-1-AP

WB analysis of A549 using 15759-1-AP

IHC staining of human liver cancer using 15759-1-AP

IHC staining of human liver cancer using 15759-1-AP

IHC staining of human kidney using 15759-1-AP

IHC staining of human kidney using 15759-1-AP

IF Staining of HeLa using 15759-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

YTHDF2 mediates the mRNA degradation of the tumor suppressors to induce AKT phosphorylation in N6-methyladenosine-dependent way in prostate cancer.

Phosphatase LHPP confers prostate cancer ferroptosis activation by modulating the AKT-SKP2-ACSL4 pathway

Correlated with better prognosis, CSTA inhibits metastasis of nasopharyngeal carcinoma cells via suppressing AKT signaling through promoting METTL3 degradation

The long intergenic noncoding RNA GAS5 reduces cisplatin-resistance in non-small cell lung cancer through the miR-217/LHPP axis.

LHPP inhibits hepatocellular carcinoma cell growth and metastasis.

Tumor suppressor LHPP regulates the proliferation of colorectal cancer cells via the PI3K/AKT pathway.