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Tested Applications
| Positive IHC detected in | human small intestine tissue, human colon tissue, human colon cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
21833-1-AP targets LGR5 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | LGR5 fusion protein Ag16389 Predict reactive species |
| Full Name | leucine-rich repeat-containing G protein-coupled receptor 5 |
| Calculated Molecular Weight | 907 aa, 100 kDa |
| GenBank Accession Number | BC096324 |
| Gene Symbol | LGR5 |
| Gene ID (NCBI) | 8549 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75473 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LGR5, also named as GPR49, GPR67 and HG38, belongs to the G-protein coupled receptor 1 family. It is an orphan receptor. LGR5 is a stem cell marker of the intestinal epithelium and the hair follicule. It is a target gene of Wnt signaling.
Publications
| Species | Application | Title |
|---|---|---|
Oncotarget LGR5 regulates pro-survival MEK/ERK and proliferative Wnt/β-catenin signalling in neuroblastoma. | ||
Inflamm Bowel Dis Overexpression of Cancer-Associated Stem Cell Gene OLFM4 in the Colonic Epithelium of Patients With Primary Sclerosing Cholangitis. | ||
J Dermatol Sci REGγ regulates hair cycle by activating Lgr5 positive hair follicle stem cells. | ||
Tumour Biol Correlation of Musashi-1, Lgr5, and pEGFR expressions in human small intestinal adenocarcinomas. | ||
Oncol Lett Detection of circulating colorectal cancer cells by a custom microfluid system before and after endoscopic metallic stent placement. | ||
Oncol Lett Expression and clinical significance of SALL4 and LGR5 in patients with lung cancer. |
