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  • Featured Product
  • KD/KO Validated

LDLR Polyclonal antibody

LDLR Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (4)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 10785-1-AP

Synonyms

LDL receptor, FHC, FH

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LDLR Polyclonal antibody (10785-1-AP)

    WB analysis using 10785-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10785-1-AP (LDLR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using LDLR Polyclonal antibody (10785-1-AP)

    WB analysis of HeLa using 10785-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10785-1-AP (LDLR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using LDLR Polyclonal antibody (10785-1-AP)

    WB analysis of HL-60 using 10785-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 10785-1-AP (LDLR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using LDLR Polyclonal antibody (10785-1-AP)

    WB analysis of human brain using 10785-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 10785-1-AP (LDLR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using LDLR Polyclonal antibody (10785-1-AP)

    WB analysis of Raji using 10785-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 10785-1-AP (LDLR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using LDLR Polyclonal antibody (10785-1-AP)

    WB analysis of mouse brain using 10785-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10785-1-AP (LDLR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using LDLR Polyclonal antibody (10785-1-AP)

    WB analysis of Jurkat using 10785-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10785-1-AP (LDLR antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using LDLR Polyclonal antibody (10785-1-AP)

    IP experiment of HeLa using 10785-1-AP

    IP result of anti-LDLR (IP:10785-1-AP, 5ug; Detection:10785-1-AP 1:500) with HeLa cells lysate 1200ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using LDLR Polyclonal antibody (10785-1-AP)

    IHC staining of mouse brain using 10785-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10785-1-AP (LDLR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using LDLR Polyclonal antibody (10785-1-AP)

    IHC staining of mouse brain using 10785-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10785-1-AP (LDLR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using LDLR Polyclonal antibody (10785-1-AP)

    IHC staining of mouse liver using 10785-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10785-1-AP (LDLR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using LDLR Polyclonal antibody (10785-1-AP)

    IHC staining of mouse liver using 10785-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10785-1-AP (LDLR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using LDLR Polyclonal antibody (10785-1-AP)

    IHC staining of human brain using 10785-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10785-1-AP (LDLR Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using LDLR Polyclonal antibody (10785-1-AP)

    IHC staining of human brain using 10785-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10785-1-AP (LDLR Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using LDLR Polyclonal antibody (10785-1-AP)

    IHC staining of human colon cancer using 10785-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10785-1-AP (LDLR antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using LDLR Polyclonal antibody (10785-1-AP)

    IHC staining of human colon cancer using 10785-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10785-1-AP (LDLR antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using LDLR Polyclonal antibody (10785-1-AP)

    IHC staining of human pancreas using 10785-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 10785-1-AP (LDLR antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using LDLR Polyclonal antibody (10785-1-AP)

    IHC staining of human pancreas using 10785-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 10785-1-AP (LDLR antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using LDLR Polyclonal antibody (10785-1-AP)

    IF Staining of HeLa using 10785-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LDLR antibody (10785-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Beta Actin antibody (66009-1-Ig, Clone: 2D4H5, red).

Tested Applications

Positive WB detected inHeLa cells, HL-60 cells, human brain tissue, Jurkat cells, mouse brain tissue, Raji cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse brain tissue, human brain tissue, mouse liver tissue, human pancreas tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10785-1-AP targets LDLR in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, pig, chicken, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LDLR fusion protein Ag1236
Full Name low density lipoprotein receptor
Calculated Molecular Weight 95 kDa
Observed Molecular Weight 100-160 kDa
GenBank Accession NumberBC014514
Gene Symbol LDLR
Gene ID (NCBI) 3949
RRIDAB_2281164
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LDLR (low density lipoprotein receptor) is a member of the LDL receptor gene family and is involved in receptor-mediated endocytosis of specific ligands. The LDLR is a cell surface glycoprotein that scavenges LDL from the blood and regulates plasma LDL cholesterol. The cytoplasmic domain of the LDL receptor is necessary for the receptor to cluster in coated pits, which promotes the rapid endocytosis of bound LDL. The protein is highly glycosylated through N- and O-linkages and thus migrates at 100 to 160 kDa bands on SDS-PAGE.

Protocols

Product Specific Protocols
WB protocol for LDLR antibody 10785-1-APDownload protocol
IHC protocol for LDLR antibody 10785-1-APDownload protocol
IF protocol for LDLR antibody 10785-1-APDownload protocol
IP protocol for LDLR antibody 10785-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Metab

Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.

Authors - Yi Fu
View Article
humanWB

Cell Metab

Identification and evaluation of a lipid-lowering small compound in preclinical models and in a Phase I trial.

Authors - Jiang Wang
View Article
human,mouseWB,IF

Sci Transl Med

Single-cell atlas of human infrapatellar fat pad and synovium implicates APOE signaling in osteoarthritis pathology

Authors - Su'an Tang
View Article
mouseWB,IP

J Clin Invest

Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation.

Authors - Shanshan Zhong
  • KO Validated
View Article
humanWB

Nat Commun

A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry.

Authors - Yunkai Zhu
View Article
mouseIF

Adv Healthc Mater

Engineered Red Blood Cell Membrane-Coating Salidroside/Indocyanine Green Nanovesicles for High-Efficiency Hypoxic Targeting Phototherapy of Triple-Negative Breast Cancer.

Authors - Yi Pan
View Article