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  • KD/KO Validated

LASS2 Polyclonal antibody

LASS2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 20344-1-AP

Synonyms

CerS2, L3, LASS2, SP260, TMSG1



Tested Applications

Positive WB detected inmouse liver tissue, HeLa cells, rat liver tissue, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20344-1-AP targets LASS2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LASS2 fusion protein Ag14151
Full Name LAG1 homolog, ceramide synthase 2
Calculated Molecular Weight 380 aa, 45 kDa
Observed Molecular Weight 45 kDa, 37 kDa
GenBank Accession NumberBC010032
Gene Symbol LASS2
Gene ID (NCBI) 29956
RRIDAB_2878677
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LASS2, also known as TMSG1, is a novel suppressor of human cancer metastasis. As one member of LASS family, including LASS1-6, LASS2 mRNA is at the highest level of all LASS members, and has the broadest tissue distribution, particularly abundant in the liver, kidney and brain in mice. The biological roles of LASS2 include protection from aging , hepatic INS resistance, and hepatocellular carcinoma (HCC) progression. LASS2 has been correlated with the degree of invasion and recurrence of carcinomas of the prostate, liver, breast and bladder.

Protocols

Product Specific Protocols
WB protocol for LASS2 antibody 20344-1-APDownload protocol
IHC protocol for LASS2 antibody 20344-1-APDownload protocol
IP protocol for LASS2 antibody 20344-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cardiovasc Res

Nogo-A reduces ceramide de novo biosynthesis to protect from heart failure.

Authors - Linda Sasset
  • KD Validated
humanWB,IHC,IF

J Cancer

LASS2 impairs proliferation of glioma stem cells and migration and invasion of glioma cells mainly via inhibition of EMT and apoptosis promotion.

Authors - Wei-Jiang Zhao
  • KD Validated
humanWB

Exp Cell Res

MicroRNA-98 promotes drug resistance and regulates mitochondrial dynamics by targeting LASS2 in bladder cancer cells.

Authors - Ting Luan
  • KD Validated
mouseWB

Toxicol Mech Methods

Chronic ethanol exposure induces cardiac fibroblast transdifferentiation via ceramide accumulation and oxidative stress

Authors - Tianyi Zhang
humanWB,IF

J Cancer

LASS2 regulates invasion and chemoresistance via ERK/Drp1 modulated mitochondrial dynamics in bladder cancer cells.

Authors - Lijuan Huang
  • KD Validated
humanWB

Tumour Biol

miR-9 promotes cell proliferation and inhibits apoptosis by targeting LASS2 in bladder cancer.

Authors - Haifeng Wang