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L1TD1 Polyclonal antibody

L1TD1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21528-1-AP

Synonyms

LINE-1 type transposase domain-containing protein 1, ES cell-associated protein 11, ES cell associated protein 11, ECAT11

Validation Data Gallery

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  • Western Blot (WB) analysis of NCCIT cells using L1TD1 Polyclonal antibody (21528-1-AP)

    WB analysis of NCCIT cells using 21528-1-AP

    WB result of L1TD1 antibody (21528-1-AP, 1:500) with NCCIT cells.

  • Immunohistochemistry (IHC) staining of human placenta tissue using L1TD1 Polyclonal antibody (21528-1-AP)

    IHC staining of human placenta using 21528-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21528-1-AP (L1TD1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using L1TD1 Polyclonal antibody (21528-1-AP)

    IHC staining of human placenta using 21528-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21528-1-AP (L1TD1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using L1TD1 Polyclonal antibody (21528-1-AP)

    IHC staining of human ovary cancer using 21528-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 21528-1-AP (L1TD1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using L1TD1 Polyclonal antibody (21528-1-AP)

    IHC staining of human ovary cancer using 21528-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 21528-1-AP (L1TD1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using L1TD1 Polyclonal antibody (21528-1-AP)

    IHC staining of human ovary tumor using 21528-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 21528-1-AP (L1TD1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using L1TD1 Polyclonal antibody (21528-1-AP)

    IHC staining of human ovary tumor using 21528-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 21528-1-AP (L1TD1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of NCCIT cells using L1TD1 Polyclonal antibody (21528-1-AP)

    IF Staining of NCCIT using 21528-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NCCIT cells using L1TD1 antibody (21528-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

Tested Applications

Positive WB detected inNCCIT cells
Positive IHC detected inhuman ovary cancer tissue, human ovary tumor tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNCCIT cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21528-1-AP targets L1TD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen L1TD1 fusion protein Ag16147
Full Name LINE-1 type transposase domain containing 1
Calculated Molecular Weight 865 aa, 99 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC111559
Gene Symbol L1TD1
Gene ID (NCBI) 54596
RRIDAB_2878878
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

L1TD1 is an RNA-binding protein highly and specifically expressed in pluripotent cells. It is essential for maintaining the pluripotentency in human embryonic stem cells (hESCs) and has been considered as a marker for undifferentiated hESCs. L1TD1 is also reported to be highly expressed in medulloblastoma cells and its expression in medulloblastoma correlates with poor clinical outcome.

Protocols

Product Specific Protocols
IHC protocol for L1TD1 antibody 21528-1-APDownload protocol
IF protocol for L1TD1 antibody 21528-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols