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Cytokeratin 15 Polyclonal antibody

Cytokeratin 15 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 10137-1-AP

Synonyms

KRT15, keratin 15, Cytokeratin-15 , Cytokeratin,Keratin, CK-15

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using Cytokeratin 15 Polyclonal antibody (10137-1-AP)

    WB analysis of A431 using 10137-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 10137-1-AP (Cytokeratin 15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using Cytokeratin 15 Polyclonal antibody (10137-1-AP)

    WB analysis of mouse thymus using 10137-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10137-1-AP (Cytokeratin 15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Cytokeratin 15 Polyclonal antibody (10137-1-AP)

    WB analysis of HeLa using 10137-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10137-1-AP (Cytokeratin 15 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using Cytokeratin 15 Polyclonal antibody (10137-1-AP)

    WB analysis of PC-3 using 10137-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 10137-1-AP (Cytokeratin 15 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A431 cells using Cytokeratin 15 Polyclonal antibody (10137-1-AP)

    IP experiment of A431 using 10137-1-AP

    IP result of anti-Cytokeratin 15 (IP:10137-1-AP, 4ug; Detection:10137-1-AP 1:8000) with A431 cells lysate 1000 ug.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Cytokeratin 15 Polyclonal antibody (10137-1-AP)

    IHC staining of human cervical cancer using 10137-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10137-1-AP (Cytokeratin 15 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Cytokeratin 15 Polyclonal antibody (10137-1-AP)

    IHC staining of human cervical cancer using 10137-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10137-1-AP (Cytokeratin 15 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Cytokeratin 15 Polyclonal antibody (10137-1-AP)

    IHC staining of human oesophagus cancer using 10137-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10137-1-AP (Cytokeratin 15 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Cytokeratin 15 Polyclonal antibody (10137-1-AP)

    IHC staining of human oesophagus cancer using 10137-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10137-1-AP (Cytokeratin 15 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cytokeratin 15 Polyclonal antibody (10137-1-AP)

    IHC staining of human breast cancer using 10137-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10137-1-AP (Cytokeratin 15 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cytokeratin 15 Polyclonal antibody (10137-1-AP)

    IHC staining of human breast cancer using 10137-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10137-1-AP (Cytokeratin 15 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Cytokeratin 15 Polyclonal antibody (10137-1-AP)

    IF Staining of HeLa using 10137-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cytokeratin 15 antibody (10137-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

Tested Applications

Positive WB detected inA431 cells, mouse thymus tissue, HeLa cells, PC-3 cells
Positive IP detected inA431 cells
Positive IHC detected inhuman cervical cancer tissue, human oesophagus cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10137-1-AP targets Cytokeratin 15 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 15 fusion protein Ag0185
Full Name keratin 15
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC002641
Gene Symbol KRT15
Gene ID (NCBI) 3866
RRIDAB_2234359
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 15 is a type I cytokeratin. It is found in some progenitor basal cells within complex epithelia.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 15 antibody 10137-1-APDownload protocol
IHC protocol for Cytokeratin 15 antibody 10137-1-APDownload protocol
IF protocol for Cytokeratin 15 antibody 10137-1-APDownload protocol
IP protocol for Cytokeratin 15 antibody 10137-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Biomater Sci

A hybrid hydrogel composed of chitin and β-glucan for the effective management of wound healing and scarring

Authors - Ximeng Kang
View Article
mouseIF

Stem Cells

LIM Homeobox Domain 2 (Lhx2) is required for corneal epithelial homeostasis.

Authors - R Sartaj
View Article
humanIF

J Invest Dermatol

GW bodies: cytoplasmic compartments in normal human skin.

Authors - Zee Jennifer M JM
View Article
humanWB

Carcinogenesis

Survival Of Skin Cancer Stem Cells Requires The Ezh2 Polycomb Group Protein.

Authors - Gautam Adhikary
View Article
mouseIF

Sci Rep

Characterization of slow cycling corneal limbal epithelial cells identifies putative stem cell markers.

Authors - R Sartaj
View Article
ratWB,IF

Front Med (Lausanne)

Metformin Promotes Mechanical Stretch-Induced Skin Regeneration by Improving the Proliferative Activity of Skin-Derived Stem Cells.

Authors - Shaoheng Xiong
View Article