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KIF27 Polyclonal antibody

KIF27 Polyclonal Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, canine

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 18833-1-AP

Synonyms

Kinesin-like protein KIF27, Kinesin like protein KIF27

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using KIF27 Polyclonal antibody (18833-1-AP)

    IF Staining of MDCK using 18833-1-AP

    Immunofluorescent analysis of MDCK cells using 18833-1-AP (KIF27 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IF/ICC detected inMDCK cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18833-1-AP targets KIF27 in IF/ICC, ELISA applications and shows reactivity with human, canine samples.

Tested Reactivity human, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name kinesin family member 27
Calculated Molecular Weight 160 kDa
GenBank Accession NumberBC140788
Gene Symbol KIF27
Gene ID (NCBI) 55582
RRIDAB_2878561
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KIF27 belongs to the kinesin-like protein family and it is a kinesin-4 motor with key roles in cytoskeletal function and ciliogenesis. KIF27 has a conserved function in microtubule organization which can influence microtubule dynamics. KIF27 is highly expressed in ciliated cells, such as olfactory sensory neurons, suggesting to be indispensable in the development and function of motile cilia. Unlike other family members, KIF27 is a slow motor due to its low phosphatase activity. (PMID: 29351996, 29450149, 21746835)

Protocols

Product Specific Protocols
IF protocol for KIF27 antibody 18833-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols