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- KD/KO Validated
KIF18A Polyclonal antibody
KIF18A Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 19245-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human colon cancer, human breast cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 8 publications below |
| IHC | See 8 publications below |
| IF | See 3 publications below |
| IP | See 1 publications below |
Product Information
19245-1-AP targets KIF18A in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KIF18A fusion protein Ag6166 |
| Full Name | kinesin family member 18A |
| Calculated Molecular Weight | 102 kDa |
| Observed Molecular Weight | 102 kDa |
| GenBank Accession Number | BC048347 |
| Gene Symbol | KIF18A |
| Gene ID (NCBI) | 81930 |
| RRID | AB_10598464 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
KIF18A belongs to the Kinesin family. KIF18A binds to microtubules and moves along them using energy from ATP hydrolysis. KIF18A is related to the localization of membrane organelles and protein complexes, as well as the maintenance of microtubule dynamics and mitosis. KIF18A expression is low in normal tissues and high in solid tumors, including breast cancer, hepatocellular carcinoma and lung adenocarcinoma, colorectal cancer, renal cell carcinoma, and head and neck squamous cell carcinoma. The molecular weight of KIF18A is 102 kDa (PMID: 36830695).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KIF18A antibody 19245-1-AP | Download protocol |
| IHC protocol for KIF18A antibody 19245-1-AP | Download protocol |
| IP protocol for KIF18A antibody 19245-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncotarget High KIF18A expression correlates with unfavorable prognosis in primary hepatocellular carcinoma. | ||
Front Genet Prognostic Biomarker KIF18A and Its Correlations With Immune Infiltrates and Mitosis in Glioma. | ||
Dis Markers Kinesin Family Member 18A (KIF18A) Contributes to the Proliferation, Migration, and Invasion of Lung Adenocarcinoma Cells In Vitro and In Vivo.
| ||
Cell Div Kif18a regulates Sirt2-mediated tubulin acetylation for spindle organization during mouse oocyte meiosis.
|
