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  • Featured Product
  • KD/KO Validated

KIF17 Polyclonal antibody

KIF17 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 14615-1-AP

Synonyms

Kinesin-like protein KIF17, KIF3X, KIF3-related motor protein, KIAA1405

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KIF17 Polyclonal antibody (14615-1-AP)

    WB analysis using 14615-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14615-1-AP (KIF17 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using KIF17 Polyclonal antibody (14615-1-AP)

    IP experiment of mouse brain using 14615-1-AP

    IP result of anti-KIF17 (IP:14615-1-AP, 4ug; Detection:14615-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using KIF17 Polyclonal antibody (14615-1-AP)

    IHC staining of human gliomas using 14615-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14615-1-AP (KIF17 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using KIF17 Polyclonal antibody (14615-1-AP)

    IHC staining of human gliomas using 14615-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14615-1-AP (KIF17 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using KIF17 Polyclonal antibody (14615-1-AP)

    IHC staining of mouse brain using 14615-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14615-1-AP (KIF17 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using KIF17 Polyclonal antibody (14615-1-AP)

    IHC staining of mouse brain using 14615-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14615-1-AP (KIF17 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using KIF17 Polyclonal antibody (14615-1-AP)

    IHC staining of human brain using 14615-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14615-1-AP (KIF17 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using KIF17 Polyclonal antibody (14615-1-AP)

    IHC staining of human brain using 14615-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14615-1-AP (KIF17 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of COS-7 cells using KIF17 Polyclonal antibody (14615-1-AP)

    IF Staining of COS-7 using 14615-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed COS-7 cells using KIF17 antibody (14615-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of COS-7 cells using KIF17 Polyclonal antibody (14615-1-AP)

    IF Staining of COS-7 using 14615-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed COS-7 cells using 14615-1-AP (KIF17 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue, human brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inCOS-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14615-1-AP targets KIF17 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity human, mouse, rat, monkey
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIF17 fusion protein Ag6217
Full Name kinesin family member 17
Calculated Molecular Weight 115 kDa
Observed Molecular Weight 170 kDa
GenBank Accession NumberBC065927
Gene Symbol KIF17
Gene ID (NCBI) 57576
RRIDAB_2131278
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for KIF17 antibody 14615-1-APDownload protocol
IHC protocol for KIF17 antibody 14615-1-APDownload protocol
IF protocol for KIF17 antibody 14615-1-APDownload protocol
IP protocol for KIF17 antibody 14615-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Dev Cell

ASK1-Mediated Phosphorylation Blocks HDAC6 Ubiquitination and Degradation to Drive the Disassembly of Photoreceptor Connecting Cilia.

Authors - Jie Ran
View Article
humanWB,IF,IP

Neurosci Bull

KIF17 Modulates Epileptic Seizures and Membrane Expression of the NMDA Receptor Subunit NR2B.

Authors - Yan Liu
View Article
humanWB,IHC,IF,CoIP

Cancer Lett

KIF17 maintains the epithelial phenotype of breast cancer cells and curbs tumour metastasis

Authors - Tianfu Li
  • KD Validated
View Article