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  • Featured Product
  • KD/KO Validated

KIF15 Polyclonal antibody

KIF15 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 55407-1-AP

Synonyms

KNSL7, KLP2, Kinesin-like protein KIF15, Kinesin-like protein 7, Kinesin-like protein 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KIF15 Polyclonal antibody (55407-1-AP)

    WB analysis using 55407-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 55407-1-AP (KIF15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using KIF15 Polyclonal antibody (55407-1-AP)

    WB analysis of HEK-293 using 55407-1-AP

    WB result of KIF15 antibody (55407-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF15 transfected HEK-293 cells.

  • Western Blot (WB) analysis of Jurkat cells using KIF15 Polyclonal antibody (55407-1-AP)

    WB analysis of Jurkat using 55407-1-AP

    WB result of KIF15 antibody (55407-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF15 transfected Jurkat cells.

  • Western Blot (WB) analysis of mouse thymus tissue using KIF15 Polyclonal antibody (55407-1-AP)

    WB analysis of mouse thymus using 55407-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 55407-1-AP (KIF15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MDA-MB-453s cells using KIF15 Polyclonal antibody (55407-1-AP)

    WB analysis of MDA-MB-453s using 55407-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 55407-1-AP (KIF15 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse thymus tissue using KIF15 Polyclonal antibody (55407-1-AP)

    IP experiment of mouse thymus using 55407-1-AP

    IP result of anti-KIF15 (IP:55407-1-AP, 4ug; Detection:55407-1-AP 1:500) with mouse thymus tissue lysate 2800ug.

  • Immunohistochemistry (IHC) staining of human testis tissue using KIF15 Polyclonal antibody (55407-1-AP)

    IHC staining of human testis using 55407-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 55407-1-AP (KIF15 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using KIF15 Polyclonal antibody (55407-1-AP)

    IHC staining of human testis using 55407-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 55407-1-AP (KIF15 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using KIF15 Polyclonal antibody (55407-1-AP)

    IF Staining of HEK-293 using 55407-1-AP

    Immunofluorescent analysis of HEK-293 cells using 55407-1-AP (KIF15 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HEK-293 cells using KIF15 Polyclonal antibody (55407-1-AP)

    FC experiment of HEK-293 using 55407-1-AP

    1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug KIF15 Polyclonal antibody (55407-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inJurkat cells, mouse thymus tissue, HEK-293 cells, MDA-MB-453s cells
Positive IP detected inmouse thymus tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55407-1-AP targets KIF15 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name kinesin family member 15
Calculated Molecular Weight 160 kDa
Observed Molecular Weight 150-165 kDa
GenBank Accession NumberNM_020242
Gene Symbol KIF15
Gene ID (NCBI) 56992
RRIDAB_11182836
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KIF15, also named as KLP2 and KNSL7, belongs to the kinesin-like protein family and KLP2 subfamily. It is a plus-end directed kinesin-like motor enzyme which involved in mitotic spindle assembly. Several isoforms of KIF15 exist due to the alternative splicing, with molecular weight between 130-160 kDa. Sometimes higher molecular weight around 200 kDa can also be observed, which may be a modified variant of KIF15. (14618103)

Protocols

Product Specific Protocols
WB protocol for KIF15 antibody 55407-1-APDownload protocol
IHC protocol for KIF15 antibody 55407-1-APDownload protocol
IF protocol for KIF15 antibody 55407-1-APDownload protocol
IP protocol for KIF15 antibody 55407-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Am J Respir Crit Care Med

Rare and Common Variants in KIF15 Contribute to Genetic Risk of Idiopathic Pulmonary Fibrosis

Authors - David Zhang
View Article
humanIHC,WB

Cancer Res

KIF15-mediated stabilization of AR and AR-V7 contributes to enzalutamide resistance in prostate cancer.

Authors - Lin Gao
  • KD Validated
View Article
humanIHC

Cancer Cell Int

Polyamine metabolism patterns characterized tumor microenvironment, prognosis, and response to immunotherapy in colorectal cancer

Authors - Enkui Zhang
View Article
humanWB,IHC

Cell Physiol Biochem

Increased KIF15 Expression Predicts a Poor Prognosis in Patients with Lung Adenocarcinoma.

Authors - Yuan Qiao
  • KD Validated
View Article
mouseWB

Front Cell Dev Biol

Transcriptional, Post-Transcriptional, and Post-Translational Mechanisms Rewrite the Tubulin Code During Cardiac Hypertrophy and Failure.

Authors - Sai Aung Phyo
View Article
humanWB,IHC

Front Oncol

KIF15 Promotes Progression of Castration Resistant Prostate Cancer by Activating EGFR Signaling Pathway.

Authors - Lin Gao
  • KD Validated
View Article