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MLK4 Polyclonal antibody

MLK4 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24395-1-AP

Synonyms

KIAA1804, mixed lineage kinase 4, MLK4, RP5 862P8.2

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using MLK4 Polyclonal antibody (24395-1-AP)

    WB analysis of HeLa using 24395-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24395-1-AP (MLK4 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of C6 cells using MLK4 Polyclonal antibody (24395-1-AP)

    IF Staining of C6 using 24395-1-AP

    Immunofluorescent analysis of C6 cells using 24395-1-AP (MLK4 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Tested Applications

Positive WB detected inHeLa cells
Positive IF/ICC detected inC6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24395-1-AP is MLK4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MLK4 fusion protein Ag18904
Full Name mixed lineage kinase 4
Calculated Molecular Weight 1036 aa, 114 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC136649
Gene Symbol MLK4
Gene ID (NCBI) 84451
RRIDAB_2879522
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MLK4 belongs to the MAP kinase kinase kinase subfamily. The MLK family members are characterized by the presence of signature sequences ofSer/Thr and Tyr kinases within their catalytic domain. The MLK1-4 contains an aminoterminal Src-Homology (SH3) domain, followed by the kinase domain, a leucine-zipper region, and a Cdc42/Rac-interactive binding (CRIB) motif. MLK4 expressed in two splicing variants, MLK4α and MLK4β. The difference of these two isoforms is located in their C-terminal portion. The negative effect of MLK4 on LPS-induced ERK and JNK activation could be very important in controlling the level of cytokine expression, and thus maintaining the balance of inflammatory responses.(PMID:21602844). This protein has 3 isoforms produced by alternative splicing with the molecular weight of 63 kDa, 113 kDa, 53 kDa.

Protocols

Product Specific Protocols
WB protocol for MLK4 antibody 24395-1-APDownload protocol
IF protocol for MLK4 antibody 24395-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols