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MRPP3 Polyclonal antibody

MRPP3 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20959-1-AP

Synonyms

KIAA0391, Protein only RNase P catalytic subunit, PRORP, Mitochondrial ribonuclease P catalytic subunit, EC:3.1.26.5

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using MRPP3 Polyclonal antibody (20959-1-AP)

    WB analysis of mouse brain using 20959-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20959-1-AP (MRPP3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using MRPP3 Polyclonal antibody (20959-1-AP)

    WB analysis of L02 using 20959-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 20959-1-AP (MRPP3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using MRPP3 Polyclonal antibody (20959-1-AP)

    WB analysis of mouse liver using 20959-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 20959-1-AP (MRPP3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using MRPP3 Polyclonal antibody (20959-1-AP)

    WB analysis of mouse brain using 20959-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20959-1-AP (MRPP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MRPP3 Polyclonal antibody (20959-1-AP)

    IF Staining of HepG2 using 20959-1-AP

    Immunofluorescent analysis of HepG2 cells, using KIAA0391 antibody 20959-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MRPP3 Polyclonal antibody (20959-1-AP)

    IF Staining of HepG2 using 20959-1-AP

    Immunofluorescent analysis of HepG2 cells, using KIAA0391 antibody 20959-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inmouse brain tissue, L02 cells, mouse liver tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

20959-1-AP targets MRPP3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRPP3 fusion protein Ag15196
Full Name KIAA0391
Calculated Molecular Weight 583 aa, 67 kDa
Observed Molecular Weight 65-67 kDa
GenBank Accession NumberBC032221
Gene Symbol MRPP3
Gene ID (NCBI) 9692
RRIDAB_10860109
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MRPP3 (Mitochondrial ribonuclease P protein 3), originally known as KIAA0391, has been identified as a member of human mitochondrial RNase P (mtRNase P), the endonuclease responsible for tRNA 5' end maturation by removing extra nucleotides from tRNA precursors at their 5' termini. Recently Daniel et al. reported that Rnase P and other mitochondrial RNA processing enzymes are localized near mt DNA nucleoids where they can initiate process of primary transcripts. (24703694)

Protocols

Product Specific Protocols
WB protocol for MRPP3 antibody 20959-1-APDownload protocol
IF protocol for MRPP3 antibody 20959-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Metab

Initial steps in RNA processing and ribosome assembly occur at mitochondrial DNA nucleoids.

Authors - Daniel F Bogenhagen
View Article