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KDSR Polyclonal antibody

KDSR Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16228-1-AP

Synonyms

3 dehydrosphinganine reductase, DHSR, FVT 1, FVT1, KDS reductase, KDSR, SDR35C1

Validation Data Gallery

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  • Western Blot (WB) analysis of human stomach tissue using KDSR Polyclonal antibody (16228-1-AP)

    WB analysis of human stomach using 16228-1-AP

    human stomach tissue were subjected to SDS PAGE followed by western blot with 16228-1-AP (KDSR antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human stomach tissue using KDSR Polyclonal antibody (16228-1-AP)

    WB analysis of human stomach using 16228-1-AP

    human stomach tissue were subjected to SDS PAGE followed by western blot with 16228-1-AP (KDSR antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using KDSR Polyclonal antibody (16228-1-AP)

    IHC staining of human placenta using 16228-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 16228-1-AP (KDSR antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using KDSR Polyclonal antibody (16228-1-AP)

    IHC staining of human placenta using 16228-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 16228-1-AP (KDSR antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inhuman stomach tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

16228-1-AP targets KDSR in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KDSR fusion protein Ag8176
Full Name 3-ketodihydrosphingosine reductase
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC008797
Gene Symbol KDSR
Gene ID (NCBI) 2531
RRIDAB_2249648
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for KDSR antibody 16228-1-APDownload protocol
IHC protocol for KDSR antibody 16228-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

Aberrant regulation of serine metabolism drives extracellular vesicle release and cancer progression

Authors - Tomofumi Yamamoto
View Article