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KCNK18 Polyclonal antibody

KCNK18 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 23712-1-AP

Synonyms

TWIK-related individual potassium channel, TRIK, TRESK2, TRESK 2, TRESK

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using KCNK18 Polyclonal antibody (23712-1-AP)

    WB analysis of A431 using 23712-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 23712-1-AP (KCNK18 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using KCNK18 Polyclonal antibody (23712-1-AP)

    WB analysis of A375 using 23712-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 23712-1-AP (KCNK18 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using KCNK18 Polyclonal antibody (23712-1-AP)

    WB analysis of MCF-7 using 23712-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 23712-1-AP (KCNK18 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using KCNK18 Polyclonal antibody (23712-1-AP)

    IF Staining of A375 using 23712-1-AP

    Immunofluorescent analysis of A375 cells using 23712-1-AP (KCNK18 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA431 cells, A375 cells, MCF-7 cells
Positive IF/ICC detected inA375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23712-1-AP targets KCNK18 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KCNK18 fusion protein Ag20514
Full Name potassium channel, subfamily K, member 18
Calculated Molecular Weight 384 aa, 44 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC152979
Gene Symbol KCNK18
Gene ID (NCBI) 338567
RRIDAB_2879309
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KCNK18, also named as TRESK and TRIK, is an outward rectifying potassium channel which produces rapidly activating outward rectifier K+ currents. It may function as background potassium channel that sets the resting membrane potential. KCNK18 is inhibited by arachidonic acid and other naturally occurring unsaturated free fatty acids. KCNK18 channel activity is enhanced by volatile anesthetics, such as isoflurane. The gene of KCNK18 maps to chromosome 10q26.11, and encodes a 384-amino acid protein with a predicted unmodified molecular weight of 44 kDa. This antibody can be used to detect endogenous KCNK18, however, the reason for the additional band of 130-140 kDa is still unknown.

Protocols

Product Specific Protocols
WB protocol for KCNK18 antibody 23712-1-APDownload protocol
IF protocol for KCNK18 antibody 23712-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols