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KCNJ1 Polyclonal antibody

KCNJ1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 20953-1-AP

Synonyms

KCNJ1, KIR1.1, ROMK, ROMK1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using KCNJ1 Polyclonal antibody (20953-1-AP)

    WB analysis of mouse brain using 20953-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20953-1-AP (KCNJ1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using KCNJ1 Polyclonal antibody (20953-1-AP)

    WB analysis of mouse brain using 20953-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20953-1-AP (KCNJ1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using KCNJ1 Polyclonal antibody (20953-1-AP)

    IP experiment of mouse brain using 20953-1-AP

    IP result of anti-KCNJ1 (IP:20953-1-AP, 4ug; Detection:20953-1-AP 1:300) with mouse brain tissue lysate 3600ug.

Tested Applications

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

The immunogen of 20953-1-AP is KCNJ1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KCNJ1 fusion protein Ag15100
Full Name potassium inwardly-rectifying channel, subfamily J, member 1
Calculated Molecular Weight 391 aa, 45 kDa
Observed Molecular Weight 75-80 kDa
GenBank Accession NumberBC074752
Gene Symbol KCNJ1
Gene ID (NCBI) 3758
RRIDAB_10732600
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KCNJ1, also called ROMK, is an inward-rectifying K+ channel responsible for basal (non-flow stimulated) K+ secretion. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium. This channel is activated by internal ATP and can be blocked by external barium. The calculated molecule weight is 45kDa, 20953-1-AP can detect the band around 75-80 kDa, it has been considered to be a dimer of KCNJ1.(PMID: 17804670; 7929082; 15767585)

Protocols

Product Specific Protocols
WB protocol for KCNJ1 antibody 20953-1-APDownload protocol
IP protocol for KCNJ1 antibody 20953-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

J Am Soc Nephrol

Activation of mTORC1 in Collecting Ducts Causes Hyperkalemia.

Authors - Zhenguo Chen
View Article

Am J Physiol Renal Physiol

(Pro)Renin Receptor Regulates Potassium Homeostasis through a Local Mechanism.

Authors - Chuanming Xu
View Article