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KCNAB1 Polyclonal antibody

KCNAB1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14697-1-AP

Synonyms

K(+) channel subunit beta-1, K(+) channel subunit beta 1, hKvBeta3, hKvb3, EC:1.1.1.-

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using KCNAB1 Polyclonal antibody (14697-1-AP)

    WB analysis of mouse heart using 14697-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 14697-1-AP (KCNAB1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Neuro-2a cells using KCNAB1 Polyclonal antibody (14697-1-AP)

    WB analysis of Neuro-2a using 14697-1-AP

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 14697-1-AP (KCNAB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using KCNAB1 Polyclonal antibody (14697-1-AP)

    IHC staining of human brain using 14697-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 14697-1-AP (KCNAB1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using KCNAB1 Polyclonal antibody (14697-1-AP)

    IHC staining of human brain using 14697-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 14697-1-AP (KCNAB1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using KCNAB1 Polyclonal antibody (14697-1-AP)

    IF Staining of HeLa using 14697-1-AP

    Immunofluorescent analysis of Hela cells, using KCNAB1 antibody 14697-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using KCNAB1 Polyclonal antibody (14697-1-AP)

    IF Staining of HeLa using 14697-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14697-1-AP (KCNAB1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse heart tissue, Neuro-2a cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1500
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14697-1-AP targets KCNAB1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KCNAB1 fusion protein Ag6339
Full Name potassium voltage-gated channel, shaker-related subfamily, beta member 1
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC043166
Gene Symbol KCNAB1
Gene ID (NCBI) 7881
RRIDAB_2131349
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for KCNAB1 antibody 14697-1-APDownload protocol
IHC protocol for KCNAB1 antibody 14697-1-APDownload protocol
IF protocol for KCNAB1 antibody 14697-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols