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JAK2 Polyclonal antibody
JAK2 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (3)
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 17670-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, NIH/3T3 cells |
| Positive IP detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 85 publications below |
| IHC | See 3 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
Product Information
The immunogen of 17670-1-AP is JAK2 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, pig, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | JAK2 fusion protein Ag11264 |
| Full Name | Janus kinase 2 (a protein tyrosine kinase) |
| Calculated Molecular Weight | 1132 aa, 130 kDa |
| Observed Molecular Weight | 150 kDa |
| GenBank Accession Number | BC039695 |
| Gene Symbol | JAK2 |
| Gene ID (NCBI) | 3717 |
| RRID | AB_2811138 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Janus kinases (JAKs) are non-receptor tyrosine kinases that are activated by cytokine binding to cytokine receptors resulting in stimulation of the JAK-STAT signaling pathway. Aberrant activation of this signaling pathway is associated with numerous immune disorders and cancer. This antibody can detect full-length band of 130 kDa, and another bands 150 kDa after posttranslational modification.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for JAK2 antibody 17670-1-AP | Download protocol |
| IHC protocol for JAK2 antibody 17670-1-AP | Download protocol |
| IP protocol for JAK2 antibody 17670-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Neuroinflammation Kynurenic acid blunts A1 astrocyte activation against neurodegeneration in HIV-associated neurocognitive disorders | ||
Cell Death Dis 4EBP1 senses extracellular glucose deprivation and initiates cell death signaling in lung cancer | ||
Theranostics Combination therapy with ropivacaine-loaded liposomes and nutrient deprivation for simultaneous cancer therapy and cancer pain relief. | ||
Arch Med Res Extracellular Vesicles from Carcinoma-associated Fibroblasts Promote EMT of Salivary Adenoid Cystic Carcinoma Via IL-6 | ||
Oxid Med Cell Longev Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation. | ||
Brain Behav Immun Interleukin-6: Its role and mechanisms in rescuing depression-like behaviors in rat models of depression. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Carly (Verified Customer) (11-17-2020) | Tested using EDTA plasma on an antibody microarray
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