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ITPA Polyclonal antibody

ITPA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16134-1-AP

Synonyms

Non-canonical purine NTP pyrophosphatase, My049, ITPase, HLC14 06 P, EC:3.6.1.9

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using ITPA Polyclonal antibody (16134-1-AP)

    WB analysis of K-562 using 16134-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using ITPA Polyclonal antibody (16134-1-AP)

    WB analysis of mouse liver using 16134-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-1080 cells using ITPA Polyclonal antibody (16134-1-AP)

    WB analysis of HT-1080 using 16134-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using ITPA Polyclonal antibody (16134-1-AP)

    WB analysis of NIH/3T3 using 16134-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using ITPA Polyclonal antibody (16134-1-AP)

    WB analysis of K-562 using 16134-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using ITPA Polyclonal antibody (16134-1-AP)

    WB analysis of K-562 using 16134-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using ITPA Polyclonal antibody (16134-1-AP)

    WB analysis of mouse liver using 16134-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using ITPA Polyclonal antibody (16134-1-AP)

    WB analysis of mouse liver using 16134-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using ITPA Polyclonal antibody (16134-1-AP)

    WB analysis of rat liver using 16134-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using ITPA Polyclonal antibody (16134-1-AP)

    WB analysis of mouse heart using 16134-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using ITPA Polyclonal antibody (16134-1-AP)

    WB analysis of human liver using 16134-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ITPA Polyclonal antibody (16134-1-AP)

    WB analysis of HeLa using 16134-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using ITPA Polyclonal antibody (16134-1-AP)

    WB analysis of mouse heart using 16134-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using ITPA Polyclonal antibody (16134-1-AP)

    IP experiment of mouse liver using 16134-1-AP

    IP result of anti-ITPA (IP:16134-1-AP, 4ug; Detection:16134-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human heart tissue using ITPA Polyclonal antibody (16134-1-AP)

    IHC staining of human heart using 16134-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16134-1-AP (ITPA Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using ITPA Polyclonal antibody (16134-1-AP)

    IHC staining of human heart using 16134-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16134-1-AP (ITPA Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using ITPA Polyclonal antibody (16134-1-AP)

    IHC staining of mouse heart using 16134-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16134-1-AP (ITPA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ITPA Polyclonal antibody (16134-1-AP)

    IHC staining of human lung cancer using 16134-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16134-1-AP (ITPA Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ITPA Polyclonal antibody (16134-1-AP)

    IHC staining of human lung cancer using 16134-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16134-1-AP (ITPA Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ITPA Polyclonal antibody (16134-1-AP)

    IF Staining of MCF-7 using 16134-1-AP

    Immunofluorescent analysis of MCF-7 cells, using ITPA antibody 11640-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inK-562 cells, HeLa cells, HT-1080 cells, human liver tissue, mouse heart tissue, mouse liver tissue, NIH/3T3 cells, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman heart tissue, human lung cancer tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16134-1-AP targets ITPA in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ITPA fusion protein Ag9128
Full Name inosine triphosphatase (nucleoside triphosphate pyrophosphatase)
Calculated Molecular Weight 194 aa, 21 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC010138
Gene Symbol ITPA
Gene ID (NCBI) 3704
RRIDAB_2129821
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ITPA(Inosine triphosphate pyrophosphatase) catalyzes the pyrophosphohydrolysis of inosine triphosphate (ITP) to inosine monophosphate (IMP).It expresses in the cytoplasm(PMID:11278832).It can be detected a band of 44 Kda as a homodimer in the western blot(PMID:19631656) though its predictedsize is 21kd.This protein has two isforms with the molecular weight of 20KDa and 21KDa.

Protocols

Product Specific Protocols
WB protocol for ITPA antibody 16134-1-APDownload protocol
IHC protocol for ITPA antibody 16134-1-APDownload protocol
IF protocol for ITPA antibody 16134-1-APDownload protocol
IP protocol for ITPA antibody 16134-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochimie

Analysis of human ITPase nucleobase specificity by site-directed mutagenesis.

Authors - Hill Amanda A
View Article
humanIHC

Oncotarget

A four-gene signature predicts survival in clear-cell renal-cell carcinoma.

Authors - Jun Dai
View Article
humanWB

Biochim Biophys Acta

Quantitative in vitro and in vivo characterization of the human P32T mutant ITPase.

Authors - Herting Greg G
View Article