K-562 cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
K-562 cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 16134-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 16134-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
human liver tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16134-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 16134-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 16134-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat liver using 16134-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
rat liver tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 16134-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
mouse heart tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 16134-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
mouse heart tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HT-1080 using 16134-1-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
HT-1080 cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 16134-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 16134-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
K-562 cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 16134-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
K-562 cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 16134-1-AP
IP result of anti-ITPA (IP:16134-1-AP, 4ug; Detection:16134-1-AP 1:1000) with mouse liver tissue lysate 4000ug.
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16134-1-AP (ITPA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 16134-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16134-1-AP (ITPA Antibody) at dilution of 1:50 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16134-1-AP (ITPA Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung cancer using 16134-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16134-1-AP (ITPA Antibody) at dilution of 1:50 (under 40x lens).
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16134-1-AP (ITPA Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of MCF-7 using 16134-1-AP
Immunofluorescent analysis of MCF-7 cells, using ITPA antibody 11640-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
K-562 cells, HeLa cells, HT-1080 cells, human liver tissue, mouse heart tissue, mouse liver tissue, NIH/3T3 cells, rat liver tissue
Positive IP detected in
mouse liver tissue
Positive IHC detected in
human heart tissue, human lung cancer tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
MCF-7 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:2000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:20-1:200
Immunofluorescence (IF)/ICC
IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
ITPA(Inosine triphosphate pyrophosphatase) catalyzes the pyrophosphohydrolysis of inosine triphosphate (ITP) to inosine monophosphate (IMP).It expresses in the cytoplasm(PMID:11278832).It can be detected a band of 44 Kda as a homodimer in the western blot(PMID:19631656) though its predictedsize is 21kd.This protein has two isforms with the molecular weight of 20KDa and 21KDa.
K-562 cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 16134-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 16134-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16134-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 16134-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 16134-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat liver using 16134-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 16134-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 16134-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HT-1080 using 16134-1-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 16134-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 16134-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 16134-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human heart using 16134-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16134-1-AP (ITPA Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 16134-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16134-1-AP (ITPA Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of mouse heart using 16134-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16134-1-AP (ITPA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 16134-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16134-1-AP (ITPA Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung cancer using 16134-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16134-1-AP (ITPA Antibody) at dilution of 1:50 (under 40x lens).
IP Figures
IP experiment of mouse liver using 16134-1-AP
IP result of anti-ITPA (IP:16134-1-AP, 4ug; Detection:16134-1-AP 1:1000) with mouse liver tissue lysate 4000ug.
IF/ICC Figures
IF Staining of MCF-7 using 16134-1-AP
Immunofluorescent analysis of MCF-7 cells, using ITPA antibody 11640-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
ITPA Polyclonal antibody
Catalog Number
16134-1-AP
Citations
3
Dilutions
WB : 1:500-1:2000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 IF/ICC : 1:10-1:100
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
