• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ITM2C Monoclonal antibody

ITM2C Monoclonal Antibody for IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2E8G11

Cat no : 60095-1-Ig

Synonyms

BRICD2C, Cerebral protein 14, E25, E25C, integral membrane protein 2C, ITM2C, ITM3, PSEC0047, Transmembrane protein BRI3

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ITM2C Monoclonal antibody (60095-1-Ig)

    IF Staining of HepG2 using 60095-1-Ig

    Immunofluorescent analysis of HepG2 cells using 60095-1-Ig (ITM2C antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG.

Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

FCSee 1 publications below

Product Information

60095-1-Ig targets ITM2C in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ITM2C fusion protein Ag4409
Full Name integral membrane protein 2C
Calculated Molecular Weight230aa,26 kDa; 267aa,30 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC025742
Gene Symbol ITM2C
Gene ID (NCBI) 81618
RRIDAB_2296429
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Integral membrane protein 2C (ITM2C) is a type II integral transmembrane protein belonging to a family composed of at least two other members, ITM2A and ITM2B. ITM2C, often called transmembrane protein BRI3, is highly expressed in brain tissue. Yeast two-hybrid screen system revealed that BRI3 could interact with beta-secretase beta-amyloid protein converting enzyme (BACE)1 and the microtubule-destabilizing protein SCG10 (STMN2). It may play a role in TNF-induced cell death and neuronal differentiation. BRI3 was found to inhibit the various processing of amyloid precursor protein (APP) by blocking the access of alpha- and beta-secretases to APP, competitive inhibition of APP processing by BRI3 may provide a new approach to Alzheimer disease (AD) therapy and prevention.

Protocols

Product Specific Protocols
IF protocol for ITM2C antibody 60095-1-IgDownload protocol
FC protocol for ITM2C antibody 60095-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanFC

NAR Genom Bioinform

Joint single cell DNA-seq and RNA-seq of gastric cancer cell lines reveals rules of in vitro evolution.

Authors - Noemi Andor
View Article