• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

IRS1 Polyclonal antibody

IRS1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (4)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Dot blot, ELISA

Conjugate

Unconjugated

Cat no : 17509-1-AP

Synonyms

IRS-1, IRS 1, insulin receptor substrate 1, HIRS 1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of A549 cells using IRS1 Polyclonal antibody (17509-1-AP)

    WB analysis of A549 using 17509-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 17509-1-AP (IRS1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using IRS1 Polyclonal antibody (17509-1-AP)

    WB analysis using 17509-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17509-1-AP (IRS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using IRS1 Polyclonal antibody (17509-1-AP)

    WB analysis of HEK-293 using 17509-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17509-1-AP (IRS1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using IRS1 Polyclonal antibody (17509-1-AP)

    WB analysis of A431 using 17509-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 17509-1-AP (IRS1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A549 cells using IRS1 Polyclonal antibody (17509-1-AP)

    IP experiment of A549 using 17509-1-AP

    IP result of anti-IRS1 (IP:17509-1-AP, 5ug; Detection:17509-1-AP 1:1000) with A549 cells lysate 3500ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using IRS1 Polyclonal antibody (17509-1-AP)

    IHC staining of human breast cancer using 17509-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 17509-1-AP (IRS1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using IRS1 Polyclonal antibody (17509-1-AP)

    IHC staining of human breast cancer using 17509-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 17509-1-AP (IRS1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using IRS1 Polyclonal antibody (17509-1-AP)

    IHC staining of human liver using 17509-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17509-1-AP (IRS1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using IRS1 Polyclonal antibody (17509-1-AP)

    IHC staining of human liver using 17509-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17509-1-AP (IRS1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using IRS1 Polyclonal antibody (17509-1-AP)

    IF Staining of A549 using 17509-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using IRS1 antibody (17509-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using IRS1 Polyclonal antibody (17509-1-AP)

    IF Staining of A549 using 17509-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using IRS1 antibody (17509-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of MCF-7 cells using IRS1 Polyclonal antibody (17509-1-AP)

    FC experiment of MCF-7 using 17509-1-AP

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human IRS1 (17509-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inA549 cells, A431 cells, HEK-293 cells, PC-3 cells
Positive IP detected inA549 cells
Positive IHC detected inhuman breast cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17509-1-AP targets IRS1 in WB, IHC, IF/ICC, FC (Intra), IP, Dot blot, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat, pig, megalobrama amblycephala
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IRS1 fusion protein Ag11714
Full Name insulin receptor substrate 1
Calculated Molecular Weight 1242 aa, 132 kDa
Observed Molecular Weight 160-185 kDa
GenBank Accession NumberBC053895
Gene Symbol IRS1
Gene ID (NCBI) 3667
RRIDAB_10596914
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ins receptor substrate 1 (IRS1) was the first cloned and characterized member of the IRS family which are involved in ins receptor (IR) and ins-like growth factor I receptor (IGF-IR) signaling. IRS1 is phosphorylated by ins receptor tyrosine kinase and is involved in various cellular processes including DNA repair fidelity, transcriptional activity, and cell growth can support tumor development and progression. Mutations in this gene are associated with type II diabetes and susceptibility to ins resistance. IRS1 has a predicted molecular weight of 132 kDa, however, as a result of its extensive serine phosphorylation it separates on a SDS gel as a band of approximately 160-185 kDa.

Protocols

Product Specific Protocols
WB protocol for IRS1 antibody 17509-1-APDownload protocol
IHC protocol for IRS1 antibody 17509-1-APDownload protocol
IF protocol for IRS1 antibody 17509-1-APDownload protocol
IP protocol for IRS1 antibody 17509-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigWB

Cell Metab

Augmentation of scleral glycolysis promotes myopia through histone lactylation

Authors - Xiaolei Lin
View Article
humanWB

Mol Cell

KARATE: PKA-induced KRAS4B-RHOA-mTORC2 supercomplex phosphorylates AKT in insulin signaling and glucose homeostasis

Authors - Hiroshi Senoo
View Article
mouseWB

Autophagy

BNIP3L/Nix-induced mitochondrial fission, mitophagy, and impaired myocyte glucose uptake are abrogated by PRKA/PKA phosphorylation.

Authors - Simone C da Silva Rosa
View Article
mouseDot blot

Environ Health Perspect

Effects of Chronic Secondhand Smoke (SHS) Exposure on Cognitive Performance and Metabolic Pathways in the Hippocampus of Wild-Type and Human Tau Mice.

Authors - Jacob Raber
View Article
mouseWB

JHEP Rep

Human umbilical cord mesenchymal stem cell-derived exosomes ameliorate liver steatosis by promoting fatty acid oxidation and reducing fatty acid synthesis

Authors - Fuji Yang
View Article
humanWB

Clin Transl Med

Aptamer-SH2 superbinder-based targeted therapy for pancreatic ductal adenocarcinoma.

Authors - An-Dong Liu
View Article