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  • Featured Product
  • KD/KO Validated

IRF3 Polyclonal antibody

IRF3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (6)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA

Conjugate

Unconjugated

Cat no : 11312-1-AP

Synonyms

IRF-3, IRF 3, interferon regulatory factor 3

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using IRF3 Polyclonal antibody (11312-1-AP)

    WB analysis of HeLa using 11312-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11312-1-AP (IRF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using IRF3 Polyclonal antibody (11312-1-AP)

    WB analysis of Jurkat cells using 11312-1-AP

    WB result of IRF3 specific antibody (11312-1-AP, 1:5000) with si-control and si-IRF3 transfected Jurkat cells.

  • Western Blot (WB) analysis of various lysates using IRF3 Polyclonal antibody (11312-1-AP)

    WB analysis using 11312-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11312-1-AP (IRF3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using IRF3 Polyclonal antibody (11312-1-AP)

    WB analysis using 11312-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11312-1-AP (IRF3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using IRF3 Polyclonal antibody (11312-1-AP)

    WB analysis using 11312-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11312-1-AP (IRF3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using IRF3 Polyclonal antibody (11312-1-AP)

    WB analysis of U-937 using 11312-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 11312-1-AP (IRF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using IRF3 Polyclonal antibody (11312-1-AP)

    WB analysis of human brain using 11312-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11312-1-AP (IRF3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using IRF3 Polyclonal antibody (11312-1-AP)

    IP experiment of Jurkat using 11312-1-AP

    IP result of anti-IRF3 (IP:11312-1-AP, 3ug; Detection:11312-1-AP 1:1000) with Jurkat cells lysate 2400ug.

  • Immunohistochemistry (IHC) staining of human spleen tissue using IRF3 Polyclonal antibody (11312-1-AP)

    IHC staining of human spleen using 11312-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 11312-1-AP (IRF3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using IRF3 Polyclonal antibody (11312-1-AP)

    IHC staining of human spleen using 11312-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 11312-1-AP (IRF3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using IRF3 Polyclonal antibody (11312-1-AP)

    IF Staining of HeLa using 11312-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IRF3 antibody (11312-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Tested Applications

Positive WB detected inHEK-293T cells, HCT 116 cells, HeLa cells, U-937 cells, human brain tissue, Jurkat cells, MCF-7 cells, MDA-MB-231 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11312-1-AP targets IRF3 in WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, rat, pig, monkey, bovine, hamster, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IRF3 fusion protein Ag1858
Full Name interferon regulatory factor 3
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 50-60 kDa
GenBank Accession NumberBC009395
Gene Symbol IRF3
Gene ID (NCBI) 3661
RRIDAB_2127004
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The virul-induced expression of interferon(IFN) genes in infected cells implicate in the interplay of several constitutively expressed and virus-activated transcription factors. A family of IFN regulatory factors(IRFs) have been shown to has a role in the transcription of IFN genes as well as IFN-stimulated genes. IRF3 is a novel key transcriptional regulator of type I IFN-dependent immune responses and involves in the innate immune response against DNA and RNA viruses, by binding to the promoters of IFN. It located in the cytoplasm of uninfected cells in an inactive form, and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, could be phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. IRF3 exists some isoforms with MV 47-49 kDa, 33 kDa and 12-16 kDa.

Protocols

Product Specific Protocols
WB protocol for IRF3 antibody 11312-1-APDownload protocol
IHC protocol for IRF3 antibody 11312-1-APDownload protocol
IF protocol for IRF3 antibody 11312-1-APDownload protocol
IP protocol for IRF3 antibody 11312-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

The Cytoplasmic DNA Sensor cGAS Promotes Mitotic Cell Death.

Authors - Christian Zierhut
View Article
humanWB,IF

Nat Immunol

The tumor suppressor PTEN has a critical role in antiviral innate immunity.

Authors - Shun Li
View Article

Mol Cell

An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation

Authors - Stephanie Pei Tung Yiu
View Article
humanWB

Cell Host Microbe

PMID: 29478775

Authors - Jiazheng Xie
View Article
humanWB,IF

Acta Pharm Sin B

Allosteric inhibition reveals SHP2-mediated tumor immunosuppression in colon cancer by single-cell transcriptomics.

Authors - Jian Gao
View Article
humanWB

Emerg Microbes Infect

NSUN2-mediated M5c methylation of IRF3 mRNA negatively regulates type I interferon responses during various viral infections

Authors - Hongyun Wang
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Sarah (Verified Customer) (12-18-2019)

This antibody is awesome- been searching for an Pab IRF 3 antibody that works well in a species other than human, and we finally found this one! Thank you!

  • Applications: Western Blot,