Filter: AllWBIHC

WB analysis of HepG2 using 60352-1-Ig
HepG2 cells were subjected to SDS PAGE followed by western blot with 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of pig liver using 60352-1-Ig
pig liver tissue were subjected to SDS PAGE followed by western blot with 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 60352-1-Ig
human testis tissue were subjected to SDS PAGE followed by western blot with 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 60352-1-Ig
L02 cells were subjected to SDS PAGE followed by western blot with 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:650 incubated at room temperature for 1.5 hours.
IHC staining of human lung cancer using 60352-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 60352-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:150 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human oesophagus cancer using 60352-1-Ig
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:150 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human oesophagus cancer using 60352-1-Ig
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:150 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 60352-1-Ig
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:150 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 60352-1-Ig
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:150 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Inhibin beta A-Specific Monoclonal antibody

Name: Inhibin beta A-Specific Monoclonal antibody
Brand: Proteintech
SKU: 60352-1-Ig

Inhibin beta A-Specific Monoclonal Antibody for WB, IHC, ELISA

Cat No. 60352-1-Ig

Clone No.1F8D8

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, ELISA

Synonyms

Activin beta A chain, EDF, FRP, INHBA, INHBA-Specific, Inhibin beta A chain, inhibin, beta A

Formulation: PBS and Azide

PBS and Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HepG2 cells, human testis tissue, L02 cells, pig liver tissue
Positive IHC detected in	human lung cancer tissue, human oesophagus cancer tissue, human stomach cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60352-1-Ig targets Inhibin beta A-Specific in WB, IHC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity	human, pig
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	inhibin, beta A
Calculated Molecular Weight	47 kDa
Observed Molecular Weight	47 kDa
GenBank Accession Number	NM_002192
Gene Symbol	Inhibin beta A
Gene ID (NCBI)	3624
RRID	AB_2881461
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P08476
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Inhibin βA(INHBA) is a ligand in the transforming growth factor β (TGF-β) superfamily. Inhibin βA forms a disulfide-linked homo-dimer known as activin A, which has been implicated in multiple biologic processes, including neoplastic progression. The inhibin βA can also bind the βB and α isoforms to form activin AB and inhibin, respectively.

Protocols

Product Specific Protocols
WB protocol for Inhibin beta A-Specific antibody 60352-1-Ig	Download protocol
IHC protocol for Inhibin beta A-Specific antibody 60352-1-Ig	Download protocol