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Human IgG4 Monoclonal antibody

Human IgG4 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1A1A2

Cat no : 66408-1-Ig

Synonyms

Human IgG4, IgG4, IGHG4

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Human IgG4 Monoclonal antibody (66408-1-Ig)

    WB analysis of human plasma using 66408-1-Ig

    human plasma (0.4 nL) were subjected to SDS PAGE followed by western blot with 66408-1-Ig (human IgG4 Antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human skeletal muscle tissue using Human IgG4 Monoclonal antibody (66408-1-Ig)

    WB analysis of human skeletal muscle using 66408-1-Ig

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66408-1-Ig (IGHG4 Antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using Human IgG4 Monoclonal antibody (66408-1-Ig)

    WB analysis of human heart using 66408-1-Ig

    human heart tissue were subjected to SDS PAGE followed by western blot with 66408-1-Ig (IGHG4 Antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Human IgG4 Monoclonal antibody (66408-1-Ig)

    IHC staining of human tonsillitis using 66408-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66408-1-Ig (Human IgG4 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Human IgG4 Monoclonal antibody (66408-1-Ig)

    IHC staining of human tonsillitis using 66408-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66408-1-Ig (Human IgG4 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human appendicitis tissue using Human IgG4 Monoclonal antibody (66408-1-Ig)

    IF Staining of human appendicitis using 66408-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using Human IgG4 antibody (66408-1-Ig, Clone: 1A1A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inhuman plasma tissue, human heart tissue, human skeletal muscle tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman appendicitis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

66408-1-Ig targets Human IgG4 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name immunoglobulin heavy constant gamma 4 (G4m marker)
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberAL928742
Gene Symbol IGHG4
Gene ID (NCBI) 3503
RRIDAB_2881780
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This antibody detects the heavy chain of human IgG4. The immunogen of this antibody is the specific domain "NKGLPSSIEK" of IGHG4.

Protocols

Product Specific Protocols
WB protocol for Human IgG4 antibody 66408-1-IgDownload protocol
IHC protocol for Human IgG4 antibody 66408-1-IgDownload protocol
IF protocol for Human IgG4 antibody 66408-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Breast Cancer Res

Clinical and biological heterogeneities in triple-negative breast cancer reveals a non-negligible role of HER2-low

Authors - Xi E Hu
View Article