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HSPA6 Polyclonal antibody
HSPA6 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (3)
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 13616-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HL-60 cells, MCF-7 cells, U-937 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 9 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
Product Information
13616-1-AP targets HSPA6 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | HSPA6 fusion protein Ag4522 |
| Full Name | heat shock 70kDa protein 6 (HSP70B') |
| Calculated Molecular Weight | 643 aa, 71 kDa |
| Observed Molecular Weight | 68-71 kDa |
| GenBank Accession Number | BC035665 |
| Gene Symbol | HSPA6 |
| Gene ID (NCBI) | 3310 |
| RRID | AB_2120122 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HSPA6 (also known as HSP70B) is a member of the HSPA (HSP70) family of heat-shock proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. Human HSP70B is known to be strictly inducible and shows very low or no basal expression levels in most cells. HSPA6 induction has been exploited as a reasonable biomarker of cellular stresses and its mRNA expression levels have been shown to be significantly induced against a variety of cellular stresses. This antibody was raised against the C-terminal region of human HSPA6 and it may cross-react with HSPA1 (HSP70), HSPA1L and HSPA2. (18229458)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HSPA6 antibody 13616-1-AP | Download protocol |
| IHC protocol for HSPA6 antibody 13616-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Mol Med ROR2 deficit may induce the tetralogy of Fallot via down-regulating of β-catenin/SOX3/HSPA6 in vitro and in vivo | ||
Am J Cancer Res The stabilization of PD-L1 by the endoplasmic reticulum stress protein GRP78 in triple-negative breast cancer. | ||
Biochim Biophys Acta Mol Cell Res Generation of a pHSPA6 gene-based multifunctional live cell sensor. | ||
Am J Physiol Heart Circ Physiol Molecular mechanisms mediating preconditioning following chronic ischemia differ from those in classical second window. | ||
Evid Based Complement Alternat Med ARHGEF10L Promotes Cervical Tumorigenesis via RhoA-Mediated Signaling. | ||
Biosci Biotechnol Biochem ARHGEF10L expression regulates cell proliferation and migration in gastric tumorigenesis. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Laureano (Verified Customer) (12-26-2019) | The antibody gave a very clean band without background
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