Various lysates were subjected to SDS PAGE followed by western blot with 21206-1-AP (HSPA4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 21206-1-AP (HSPA4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 21206-1-AP
WB result of HSPA4 antibody (21206-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSPA4 transfected HEK-293 cells.
WB result of HSPA4 antibody (21206-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSPA4 transfected HEK-293 cells.
WB analysis of mouse testis using 21206-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 21206-1-AP (HSPA4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
mouse testis tissue were subjected to SDS PAGE followed by western blot with 21206-1-AP (HSPA4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 21206-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21206-1-AP (HSPA4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21206-1-AP (HSPA4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 21206-1-AP
IP result of anti-HSPA4 (IP:21206-1-AP, 4ug; Detection:21206-1-AP 1:1000) with mouse brain tissue lysate 4000ug.
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 21206-1-AP (HSPA4 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HepG2 using 21206-1-AP
Immunofluorescent analysis of HepG2 cells, using HSPA4 antibody 21206-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Immunofluorescent analysis of HepG2 cells, using HSPA4 antibody 21206-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IF Staining of MCF-7 using 21206-1-AP
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using HSPA4 antibody (21206-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using HSPA4 antibody (21206-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of C6 using 21206-1-AP
1x10^6 C6 cells were intracellularly stained with 0.4 ug HSPA4 Polyclonal antibody (21206-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1x10^6 C6 cells were intracellularly stained with 0.4 ug HSPA4 Polyclonal antibody (21206-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human colon cancer tissue, human breast cancer tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
MCF-7 cells, HepG2 cells
Positive FC (Intra) detected in
C6 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:2000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:20-1:200
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
HSPA4 (also known as Apg-2, HSP70RY) is a 110 kDa cytosolic protein, a member of the heat-shock protein 110 (HSP110) subfamily of HSP70 proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. HSPA4 is ubiquitously expressed and its expression is not heat inducible. Overexpression of HSPA4 has been reported in some leukemia and solid tumors. This antibody well recognized the endogenous HSPA4 protein in mouse brain/ testis tissues. (PMID: 21487003)
Folate deprivation induces cell cycle arrest at G0/G1 phase and apoptosis in hippocampal neuron cells through down-regulation of IGF-1 signaling pathway.
Various lysates were subjected to SDS PAGE followed by western blot with 21206-1-AP (HSPA4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 21206-1-AP
WB result of HSPA4 antibody (21206-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSPA4 transfected HEK-293 cells.
WB analysis of mouse testis using 21206-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 21206-1-AP (HSPA4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 21206-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21206-1-AP (HSPA4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human colon cancer using 21206-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 21206-1-AP (HSPA4 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human colon cancer using 21206-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 21206-1-AP (HSPA4 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human breast cancer using 21206-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 21206-1-AP (HSPA4 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human breast cancer using 21206-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 21206-1-AP (HSPA4 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human pancreas cancer using 21206-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 21206-1-AP (HSPA4 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human pancreas cancer using 21206-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 21206-1-AP (HSPA4 antibody) at dilution of 1:100 (under 40x lens).
IP Figures
IP experiment of mouse brain using 21206-1-AP
IP result of anti-HSPA4 (IP:21206-1-AP, 4ug; Detection:21206-1-AP 1:1000) with mouse brain tissue lysate 4000ug.
IF/ICC Figures
IF Staining of HepG2 using 21206-1-AP
Immunofluorescent analysis of HepG2 cells, using HSPA4 antibody 21206-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IF Staining of MCF-7 using 21206-1-AP
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using HSPA4 antibody (21206-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC (INTRA) Figures
FC experiment of C6 using 21206-1-AP
1x10^6 C6 cells were intracellularly stained with 0.4 ug HSPA4 Polyclonal antibody (21206-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
HSPA4 Polyclonal antibody
Catalog Number
21206-1-AP
Citations
4
Dilutions
WB : 1:500-1:2000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 IF/ICC : 1:50-1:500 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Reactivity
human, mouse, rat, monkey
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
