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HPS3 Polyclonal antibody

HPS3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10768-1-AP

Synonyms

Hermansky-Pudlak syndrome 3 protein, BLOC-2 complex member HPS3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using HPS3 Polyclonal antibody (10768-1-AP)

    WB analysis of HeLa using 10768-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10768-1-AP (HPS3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293T cells using HPS3 Polyclonal antibody (10768-1-AP)

    WB analysis of HEK-293T using 10768-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 10768-1-AP (HPS3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using HPS3 Polyclonal antibody (10768-1-AP)

    WB analysis of HEK-293 using 10768-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10768-1-AP (HPS3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HPS3 Polyclonal antibody (10768-1-AP)

    IHC staining of human colon cancer using 10768-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10768-1-AP (HPS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HPS3 Polyclonal antibody (10768-1-AP)

    IF Staining of HeLa using 10768-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using HPS3 antibody (10768-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HEK-293T cells, HEK-293 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

10768-1-AP targets HPS3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HPS3 fusion protein Ag1209
Full Name Hermansky-Pudlak syndrome 3
Calculated Molecular Weight114 kDa
Observed Molecular Weight 113 kDa
GenBank Accession NumberBC022062
Gene Symbol HPS3
Gene ID (NCBI) 84343
RRIDAB_2120571
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for HPS3 antibody 10768-1-APDownload protocol
IHC protocol for HPS3 antibody 10768-1-APDownload protocol
IF protocol for HPS3 antibody 10768-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS One

Cellular and molecular defects in a patient with Hermansky-Pudlak syndrome type 5.

Authors - Joshi Stephen
View Article