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HP1BP3 Polyclonal antibody

HP1BP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 24556-1-AP

Synonyms

Protein HP1-BP74, Protein HP1 BP74, HP1 BP74, Heterochromatin protein 1-binding protein 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HP1BP3 Polyclonal antibody (24556-1-AP)

    WB analysis using 24556-1-AP

    Mouse thymus lysates were subjected to SDS PAGE followed by western blot with 24556-1-AP (HP1BP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using HP1BP3 Polyclonal antibody (24556-1-AP)

    WB analysis of HeLa using 24556-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24556-1-AP (HP1BP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using HP1BP3 Polyclonal antibody (24556-1-AP)

    WB analysis of MCF-7 using 24556-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 24556-1-AP (HP1BP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using HP1BP3 Polyclonal antibody (24556-1-AP)

    WB analysis of mouse thymus using 24556-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 24556-1-AP (HP1BP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using HP1BP3 Polyclonal antibody (24556-1-AP)

    IP experiment of HeLa using 24556-1-AP

    IP result of anti-HP1BP3 (IP:24556-1-AP, 4ug; Detection:24556-1-AP 1:300) with HeLa cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using HP1BP3 Polyclonal antibody (24556-1-AP)

    IHC staining of human skin cancer using 24556-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 24556-1-AP (HP1BP3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HP1BP3 Polyclonal antibody (24556-1-AP)

    IF Staining of HeLa using 24556-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HP1BP3 antibody (24556-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

Tested Applications

Positive WB detected inmouse thymus tissue, HeLa cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

24556-1-AP targets HP1BP3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HP1BP3 fusion protein Ag18709
Full Name heterochromatin protein 1, binding protein 3
Calculated Molecular Weight 553 aa, 61 kDa
Observed Molecular Weight 70-75 kDa
GenBank Accession NumberBC045660
Gene Symbol HP1BP3
Gene ID (NCBI) 50809
RRIDAB_2879606
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Heterochromatin protein 1 binding protein 3(HP1BP3) associates with chromatin throughout the cell cycle and is required to maintain higher order chromatin structure during metaphase. HP1BP3 as a novel modulator of cognitive aging using a genetically diverse population of mice and confirm that HP1BP3 protein levels are significantly reduced in the hippocampi of cognitively impaired elderly humans relative to cognitively intact controls. Double-band staining is typical of HP1BP3 expression, indicative of multiple splice variants present in brain tissue.

Protocols

Product Specific Protocols
WB protocol for HP1BP3 antibody 24556-1-APDownload protocol
IHC protocol for HP1BP3 antibody 24556-1-APDownload protocol
IF protocol for HP1BP3 antibody 24556-1-APDownload protocol
IP protocol for HP1BP3 antibody 24556-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Neurobiol Aging

Systems genetics identifies Hp1bp3 as a novel modulator of cognitive aging.

Authors - Sarah M Neuner
View Article