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  • Featured Product
  • KD/KO Validated

HOMER1 Polyclonal antibody

HOMER1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 12433-1-AP

Synonyms

HOMER1A, Homer-1, Homer protein homolog 1, homer homolog 1 (Drosophila), Homer 1

Validation Data Gallery

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  • Western Blot (WB) analysis of rat brain tissue using HOMER1 Polyclonal antibody (12433-1-AP)

    WB analysis of rat brain using 12433-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 12433-1-AP (HOMER1 antibody) at dilution of 1:5000 incubated at room temperature for 1 hour.

  • Western Blot (WB) analysis of human/mouse brain using HOMER1 Polyclonal antibody (12433-1-AP)

    WB analysis of human/mouse brain using 12433-1-AP

    WB result of anti-HOMER1 (12433-1-ap) with mouse brain lysate and human brain lysate.

  • Western Blot (WB) analysis of NIH/3T3 cells using HOMER1 Polyclonal antibody (12433-1-AP)

    WB analysis of NIH/3T3 using 12433-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12433-1-AP (HOMER1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of C2C12 cells using HOMER1 Polyclonal antibody (12433-1-AP)

    WB analysis of C2C12 using 12433-1-AP

    C2C12 cell were subjected to SDS PAGE followed by western blot with 12433-1-AP (HOMER1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using HOMER1 Polyclonal antibody (12433-1-AP)

    WB analysis of human brain using 12433-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12433-1-AP (HOMER1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of fetal human brain tissue using HOMER1 Polyclonal antibody (12433-1-AP)

    IP experiment of fetal human brain using 12433-1-AP

    IP result of anti-HOMER1 (IP:12433-1-AP, 4ug; Detection:12433-1-AP 1:1000) with fetal human brain tissue lysate 4000 ug.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using HOMER1 Polyclonal antibody (12433-1-AP)

    IHC staining of human gliomas using 12433-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12433-1-AP (HOMER1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using HOMER1 Polyclonal antibody (12433-1-AP)

    IHC staining of human gliomas using 12433-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12433-1-AP (HOMER1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using HOMER1 Polyclonal antibody (12433-1-AP)

    IHC staining of mouse brain using 12433-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12433-1-AP (HOMER1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using HOMER1 Polyclonal antibody (12433-1-AP)

    IHC staining of mouse brain using 12433-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12433-1-AP (HOMER1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using HOMER1 Polyclonal antibody (12433-1-AP)

    IHC staining of human pancreas using 12433-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12433-1-AP (HOMER1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using HOMER1 Polyclonal antibody (12433-1-AP)

    IHC staining of human pancreas using 12433-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12433-1-AP (HOMER1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using HOMER1 Polyclonal antibody (12433-1-AP)

    IHC staining of mouse brain using 12433-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12433-1-AP (HOMER1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using HOMER1 Polyclonal antibody (12433-1-AP)

    IHC staining of mouse brain using 12433-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12433-1-AP (HOMER1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using HOMER1 Polyclonal antibody (12433-1-AP)

    IHC staining of rat brain using 12433-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12433-1-AP (HOMER1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using HOMER1 Polyclonal antibody (12433-1-AP)

    IHC staining of rat brain using 12433-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12433-1-AP (HOMER1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using HOMER1 Polyclonal antibody (12433-1-AP)

    IF Staining of rat brain using 12433-1-AP

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 12433-1-AP (HOMER1 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using HOMER1 Polyclonal antibody (12433-1-AP)

    IF Staining of PC-12 using 12433-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using HOMER1 antibody (12433-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using HOMER1 Polyclonal antibody (12433-1-AP)

    IF Staining of MCF-7 using 12433-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 12433-1-AP (HOMER1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of SH-SY5Y cells using HOMER1 Polyclonal antibody (12433-1-AP)

    FC experiment of SH-SY5Y using 12433-1-AP

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human HOMER1 (12433-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inrat brain tissue, human brain tissue, human/mouse brain
Positive IP detected infetal human brain tissue
Positive IHC detected inhuman gliomas tissue, mouse brain tissue, rat brain tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue
Positive IF/ICC detected inPC-12 cells, MCF-7 cells
Positive FC (Intra) detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12433-1-AP targets HOMER1 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat, zebra finches
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HOMER1 fusion protein Ag3109
Full Name homer homolog 1 (Drosophila)
Calculated Molecular Weight 40 kDa
Observed Molecular Weight 46-48 kDa
GenBank Accession NumberBC015502
Gene Symbol HOMER1
Gene ID (NCBI) 9456
RRIDAB_2295573
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human HOMER1, also named as SYN47, belongs to the Homer family. Human HOMER1 has some isoforms: HOMER1a, HOMER1b, HOMER1c, HOMER1e and HOMER1h. Mouse Homer1 also named as Vesl-1 and it has some isoforms: Vesl-1l, Vesl-1m, Vesl-1s, Homer1b, and Homer1d. HOMER1 is a postsynaptic density scaffolding protein. It is a neuronal immediate-early gene (IEG) that is enriched at excitatory synapses and binds mGluRs. In WB, the MW of HOMER1 is about 46-50kd, 40-43kd and 20-26kd.

Protocols

Product Specific Protocols
WB protocol for HOMER1 antibody 12433-1-APDownload protocol
IHC protocol for HOMER1 antibody 12433-1-APDownload protocol
IF protocol for HOMER1 antibody 12433-1-APDownload protocol
IP protocol for HOMER1 antibody 12433-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Psychiatry

A recurrent SHANK1 mutation implicated in autism spectrum disorder causes autistic-like core behaviors in mice via downregulation of mGluR1-IP3R1-calcium signaling.

Authors - Yue Qin
View Article
mouseWB

Elife

Pathway-specific dysregulation of striatal excitatory synapses by LRRK2 mutations.

Authors - Chuyu Chen
View Article
mouseIF

PLoS Genet

Cell fate specification in the lingual epithelium is controlled by antagonistic activities of Sonic hedgehog and retinoic acid.

Authors - Maha El Shahawy
View Article
ratIF

Aging (Albany NY)

Liraglutide preconditioning attenuates myocardial ischemia/ reperfusion injury via homer1 activation.

Authors - Xiangrong Cui
  • KD Validated
View Article
Mouse, RatWB

CNS Neurosci Ther

Acid-sensing Ion Channels Activation and Hypoxia Upregulate Homer1a Expression.

Authors - Jing-Jing Su
View Article
mouseWB,IF

Cell Signal

Tissue kallikrein protects neurons from hypoxia/reoxygenation-induced cell injury through Homer1b/c.

Authors - Su Jingjing J
View Article