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- KD/KO Validated
HIF-1 alpha Monoclonal antibody
HIF-1 alpha Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (1)
Applications
WB, IHC, IF, IP, ChIP, ELISA
Conjugate
Unconjugated
CloneNo.
1H3C12
Cat no : 66730-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, Cobalt Chloride treated HeLa cells, Cobalt Chloride treated HepG2 cells |
Samples need to be treated with hypoxia stimulation.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 44 publications below |
| IHC | See 14 publications below |
| IF | See 12 publications below |
| IP | See 2 publications below |
| ChIP | See 1 publications below |
Product Information
66730-1-Ig targets HIF-1 alpha in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | HIF-1 alpha fusion protein Ag15198 |
| Full Name | hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) |
| Calculated Molecular Weight | 826 aa, 93 kDa |
| Observed Molecular Weight | 120 kDa |
| GenBank Accession Number | BC012527 |
| Gene Symbol | HIF1A |
| Gene ID (NCBI) | 3091 |
| RRID | AB_2882080 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918). .
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HIF-1 alpha antibody 66730-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Transgelin Promotes Glioblastoma Stem Cell Hypoxic Responses and Maintenance Through p53 Acetylation | ||
Theranostics ITGB2-mediated metabolic switch in CAFs promotes OSCC proliferation by oxidation of NADH in mitochondrial oxidative phosphorylation system. | ||
Oxid Med Cell Longev Chitosan Oligosaccharides Alleviate H2O2-stimulated Granulosa Cell Damage via HIF-1α Signaling Pathway. | ||
J Med Chem Potent Inhibition of HIF1α and p300 Interaction by a Constrained Peptide Derived from CITED2. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH S (Verified Customer) (05-16-2024) | Excellent
 |
FH balawant (Verified Customer) (12-20-2022) | I have used this antibody for western blot and it is working great
|
FH Diane (Verified Customer) (02-02-2021) | incubated over night 4 degrees C. 1:2500 HRP conjugated secondary. Opti-4CN substrate kit for detection. Also tried ICC on cytospins so ran out of trial size before fully optimized but has potential to provide the information that I am needing.
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