HEXIM1 AntibodyRabbit Polyclonal
|Tested applications||ELISA, WB, IHC, IF, IP|
|Cited applications||CoIP, IF, IHC, IP, WB|
|Species specificity||Human,Mouse,Rat; other species not tested.|
|Cited species||human, mouse|
|Positive WB detected in||HeLa cells, human brain tissue, MCF7 cells|
|Positive IP detected in||MCF-7 cells|
|Positive IHC detected in||human breast cancer tissue, human cervical cancer tissue, human placenta tissue|
|Positive IF detected in||MCF-7 cells|
|Recommended dilution||WB: 1:200-1:2000|
IP = Immunoprecipitation
- HeLa cells were subjected to SDS PAGE followed by western blot with 15676-1-AP(HEXIM1 antibody) at dilution of 1:1500
- Immunohistochemical of paraffin-embedded human breast cancer using 15676-1-AP(HEXIM1 antibody) at dilution of 1:50 (under 40x lens)
- Immunofluorescent analysis of MCF-7 cells, using HEXIM1 antibody 15676-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- IP Result of anti-HEXIM1 (IP:15676-1-AP, 4ug; Detection:15676-1-AP 1:500) with MCF-7 cells lysate 2000ug.
|Source||Rabbit||Purification method||Antigen affinity purification|
|Isotype||IgG||Storage||PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.|
|Immunogen||HEXIM1 fusion protein ag8144||Full name||hexamethylene bis-acetamide inducible 1|
|BC006460||Gene ID (NCBI)||10614|
|Gene symbol||HEXIM1||Synonyms||Cardiac lineage protein 1, CLP1, EDG1, FLJ13562, HEXIM1, HIS1, MAQ1, Menage a quatre protein 1, Protein HEXIM1|
HEXIM1, also named CLP1, EDG1, HIS1 or MAQ1, is best known as the inhibitor of positive transcription elongation factor b, which regulates the transcription elongation of RNA polymerase II and controls 60-70% of mRNA synthesis. HEXIM1 regulates NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. Previous studies showed that HEXIM1 interacts with two key p53 regulators, nucleophosmin and HDM2, implying a possible connection between HEXIM1 and the p53 signaling pathway. For this antibody, western blotting analysis always appears a 70-kDa band which is consistent with that in a previous finding (PMID:15713662) with a currently unknown reason.