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HEBP1 Polyclonal antibody

HEBP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16842-1-AP

Synonyms

HBP, HEBP, HEBP1, heme binding protein 1, p22HBP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HEBP1 Polyclonal antibody (16842-1-AP)

    WB analysis using 16842-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16842-1-AP (HEBP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using HEBP1 Polyclonal antibody (16842-1-AP)

    WB analysis of A549 using 16842-1-AP

    WB result of HEBP1 antibody (16842-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HEBP1 transfected A549 cells.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HEBP1 Polyclonal antibody (16842-1-AP)

    IHC staining of human liver cancer using 16842-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16842-1-AP (HEBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HEBP1 Polyclonal antibody (16842-1-AP)

    IHC staining of human liver cancer using 16842-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16842-1-AP (HEBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inL02 cells, A549 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16842-1-AP targets HEBP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HEBP1 fusion protein Ag10538
Full Name heme binding protein 1
Calculated Molecular Weight 189 aa, 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC016277
Gene Symbol HEBP1
Gene ID (NCBI) 50865
RRIDAB_2117378
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HEBP1 (heme binding protein 1) is an intracellular tetrapyrrole-binding protein. Catalog#16842-1-AP is a rabbit polyclonal antibody raised against the full-length of human HEBP1. The MW of this protein is 21 kDa, and this antibody specially recognises the 21 kDa protein.

Protocols

Product Specific Protocols
WB protocol for HEBP1 antibody 16842-1-APDownload protocol
IHC protocol for HEBP1 antibody 16842-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols