TIM3 Polyclonal antibody
TIM3 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 11872-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | PC-3 cells, Daudi cells, Raji cells, RAW 264.7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 4 publications below |
IHC | See 2 publications below |
Product Information
11872-1-AP targets TIM3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | TIM3 fusion protein Ag2459 |
Full Name | hepatitis A virus cellular receptor 2 |
Calculated Molecular Weight | 301 aa, 33 kDa |
Observed Molecular Weight | 50-70 kDa, 33 kDa |
GenBank Accession Number | BC020843 |
Gene Symbol | TIM3 |
Gene ID (NCBI) | 84868 |
RRID | AB_10734330 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TIM3, also known as HAVCR2, is a member of the recently discovered T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection (PMID: 23180819). TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity (PMID: 16286920). TIM3 is a 280-aa membrane protein with a calculated molecular weight of 33 kDa, the higher molecular weights between 50 and 70 kDa probably represent glycosylated TIM3 (PMID: 20107545; 17069754; 11725301)
Protocols
Product Specific Protocols | |
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WB protocol for TIM3 antibody 11872-1-AP | Download protocol |
FC protocol for TIM3 antibody 11872-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Front Mol Neurosci Galectin-9/Tim-3 pathway mediates dopaminergic neurodegeneration in MPTP-induced mouse model of Parkinson’s disease | ||
Front Cell Infect Microbiol The Role of Tim-3 on dNK Cells Dysfunction During Abnormal Pregnancy With Toxoplasma gondii Infection. | ||
CNS Neurosci Ther PTX3 mediates the infiltration, migration, and inflammation-resolving-polarization of macrophages in glioblastoma. | ||
Iran J Immunol The Immunomodulatory Effects and Mechanisms of Tim-3 Action in the Early Stage of Mice with Severe Acute Pancreatitis. | ||
Immun Inflamm Dis Galectin-9 and myeloid-derived suppressor cell as prognostic indicators for chronic lymphocytic leukemia |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Qing (Verified Customer) (06-10-2022) | It work nicely
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