• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

HACL1 Polyclonal antibody

HACL1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 15519-1-AP

Synonyms

2 HPCL, 2 hydroxyacyl CoA lyase 1, 2 hydroxyphytanoyl CoA lyase, HACL1, HPCL, HPCL2, PHYH2, Phytanoyl CoA 2 hydroxylase 2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using HACL1 Polyclonal antibody (15519-1-AP)

    WB analysis of HeLa using 15519-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15519-1-AP (HACL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using HACL1 Polyclonal antibody (15519-1-AP)

    WB analysis of HEK-293 using 15519-1-AP

    WB result of HACL1 antibody (15519-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HACL1 transfected HEK-293 cells.

  • Western Blot (WB) analysis of HEK-293 cells using HACL1 Polyclonal antibody (15519-1-AP)

    WB analysis of HEK-293 using 15519-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15519-1-AP (HACL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using HACL1 Polyclonal antibody (15519-1-AP)

    IP experiment of HeLa using 15519-1-AP

    IP result of anti-HACL1 (IP:15519-1-AP, 4ug; Detection:15519-1-AP 1:300) with HeLa cells lysate 2640 ug.

  • Immunohistochemistry (IHC) staining of rat liver tissue using HACL1 Polyclonal antibody (15519-1-AP)

    IHC staining of rat liver using 15519-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 15519-1-AP (HACL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat liver tissue using HACL1 Polyclonal antibody (15519-1-AP)

    IHC staining of rat liver using 15519-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 15519-1-AP (HACL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using HACL1 Polyclonal antibody (15519-1-AP)

    IHC staining of rat kidney using 15519-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney using 15519-1-AP (HACL1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using HACL1 Polyclonal antibody (15519-1-AP)

    IHC staining of human liver using 15519-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15519-1-AP (HACL1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using HACL1 Polyclonal antibody (15519-1-AP)

    IHC staining of human liver using 15519-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15519-1-AP (HACL1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of rat liver tissue using HACL1 Polyclonal antibody (15519-1-AP)

    IHC staining of rat liver using 15519-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver using 15519-1-AP (HACL1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of rat liver tissue using HACL1 Polyclonal antibody (15519-1-AP)

    IHC staining of rat liver using 15519-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver using 15519-1-AP (HACL1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using HACL1 Polyclonal antibody (15519-1-AP)

    IHC staining of rat kidney using 15519-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney using 15519-1-AP (HACL1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of rat liver tissue using HACL1 Polyclonal antibody (15519-1-AP)

    IHC staining of rat liver using 15519-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver slide using 15519-1-AP (HACL1 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of rat liver tissue using HACL1 Polyclonal antibody (15519-1-AP)

    IHC staining of rat liver using 15519-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver slide using 15519-1-AP (HACL1 Antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HACL1 Polyclonal antibody (15519-1-AP)

    IF Staining of HeLa using 15519-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15519-1-AP (HACL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells
Positive IP detected inHeLa cells
Positive IHC detected inrat liver tissue, human liver tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15519-1-AP targets HACL1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HACL1 fusion protein Ag7830
Full Name 2-hydroxyacyl-CoA lyase 1
Calculated Molecular Weight 64 kDa
Observed Molecular Weight 64 kDa
GenBank Accession NumberBC001627
Gene Symbol HACL1
Gene ID (NCBI) 26061
RRIDAB_2279502
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for HACL1 antibody 15519-1-APDownload protocol
IHC protocol for HACL1 antibody 15519-1-APDownload protocol
IF protocol for HACL1 antibody 15519-1-APDownload protocol
IP protocol for HACL1 antibody 15519-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols