HA-tag AntibodyRabbit Polyclonal
|Tested applications||ELISA, WB, IP, IF|
|Cited applications||IF, IP, WB|
|Species specificity||Recombinant protein, Human, Mouse, Rat; other species not tested.|
|Positive WB detected in||recombinant protein|
|Positive IP detected in||Transfected HEK-293 cells|
|Positive IF detected in||Transfected HEK-293 cells|
|Recommended dilution||WB: 1:1000-1:10000|
IP = Immunoprecipitation
- Western blot of HA-tagged fusion protein with anti-HA-tag (51064-2-AP) at various dilutions.
- IP Result of anti-HA-tag (IP:51064-2-AP, 3ug; Detection:51064-2-AP 1:2000) with Transfected HEK-293 cells lysate 500ug.
- Immunofluorescent analysis of Transfected HEK-293 cells using 51064-2-AP( HA-tag Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
|Source||Rabbit||Purification method||Antigen affinity purification|
|Isotype||IgG||Storage||PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.|
|Gene ID (NCBI)|
|Gene symbol||Synonyms||CYPYDVPDYA, HA tag|
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The HA tag is corresponds to amino acid residues YPYDVPDYA of human influenza virus hemagglutinin(HA). Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. The HA tag is useful in western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-tag.